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30558

Sigma-Aldrich

Abberior® STAR 635, NHS ester

for STED application

Synonym(s):

super-resolution Microscopy

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

Quality Level

concentration

≥50.0% (degree of coupling)

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)

Application

  • Abberior STAR 635 goat anti-rabbit antibody has been used for STED (stimulated emission depletion) imaging of primary cultured striatal neurons obtained from rats.
  • Abberior STAR 635 anti-rabbit antibody has been used for STED imaging of non-sensory supporting cells obtained from cochlea of rats and mice.
  • Abberior STAR 635 conjugated with secondary antibody has been used for STED imaging of microtubules in cells.

Suitability

Designed and tested for fluorescent super-resolution microscopy

Legal Information

6538 is a trademark of American Type Culture Collection
abberior is a registered trademark of Abberior GmbH

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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