11745832910
Roche
DIG-High Prime DNA Labeling and Detection Starter Kit I
sufficient for 12 labeling reactions, sufficient for 24 blots, suitable for hybridization, suitable for Northern blotting, suitable for Southern blotting
Synonym(s):
DIG system, dna labeling
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About This Item
UNSPSC Code:
41105500
Recommended Products
usage
sufficient for 12 labeling reactions
sufficient for 24 blots
Quality Level
packaging
kit of 1 (7 components)
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
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technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
greener alternative category
, Aligned
storage temp.
−20°C
General description
The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking solution, combined stock solution of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent. The sample material can be: DNA fragments of at least 100bp, linearized plasmid, cosmid or λDNA, or supercoiled DNA. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand. This is a convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG-High Prime DNA Labeling and Detection Starter Kit I has been used in a variety of hybridization techniques:
- in Southern blots
- in northern blots
- in dot blots
- in colony and plaque hybridizations
- for all types of filter hybridization
- for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley and wheat
Features and Benefits
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 principles of greener chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG system, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Packaging
1 kit containing 7 components.
Quality
Function tested in a dot blot.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- DIG-High Prime 5x concentrated
- DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml
- DNA Dilution Buffer
- Anti-Digoxigenin-AP Conjugate antibody
- NBT/BCIP Stock Solution, concentrated
- Blocking Solution 10x concentrated
- DIG Easy Hyb Granules
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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