TGFBMAG-64K-01

MILLIPLEX^® TGFß1 Magnetic Bead Single Plex Kit - Immunology Multiplex Assay

This Immunology Multiplex Assay is used for the simultaneous quantification of the TGF-β1 biomarker in the following species: Mouse/Human/Rat/Pig/Horse/Rabbit/Guinea Pig/Hamster.

• Synonym(s): Luminex^® cytokine immunoassay, Millipore TGF beta kit, transforming growth factor beta 1 kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: rat, canine, feline, nonhuman primates, mouse, human, pig
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 100%; sensitivity: 12 pg/mL; standard curve range: 9.8-2,500 pg/mL
• technique(s): multiplexing: suitable
• compatibility: configured for Single Plex
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

The transforming growth factor beta (TGFβ) system, a superfamily of cytokines as well as signaling pathways, is highly conserved throughout the animal kingdom. TGFβ functions in immune cell development, angiogenesis, wound healing and embryonic development, bone development and plays a critical role in immune response, heart disease, and cancer. In its normal state TGFβ is one of the few classes of proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGFβ signaling pathway result in tumor cell resistance to the effects of normally functioning TGFβ, causing proliferation without regulation. Initial research suggests that VE-cadherin may enhance the mutated TGFβ signaling pathway.

The MILLIPLEX^® TGFβ1 Single Plex Magnetic Bead Kit is a single plex kit to be used for the quantification of TGFβ in serum, plasma, tissue/cell lysate and culture supernatant
samples.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

In addition to human TGFβ1, the assay can detect TGFβ1 in serum of the following; horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgous monkey. However, the exact amount of cross reactivity has not been determined.

Application

• Analyte: TGFβ1
• Recommended Species: In addition to human TGFβ1, the assay can detect TGFβ1 in serum of the following: horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgus monkey. However, the exact amount of cross reactivity has not been determined.
• Recommended Sample Type: Serum, plasma, cell/tissue culture supernatants and lysates
• NOTE: This assay requires a sample acidification treatment using the provided reagents and protocol.
• Recommended Sample Dilution: 25 μL per well of a 1:30 dilution after acidification treatment of serum and plasma samples; cell/tissue culture samples may require dilution prior to acidification treatment (see protocol for details).
• Assay Run Time: Overnight (16-18 hours) at 2-8°C or alternatively, 2 hours at room temperature (20-25°C).
• Research Category: Inflammation & Immunology
• Research Subcategory: Cardiovascular Disease, Chronic Inflammatory Disease, Immune Disorders, Inflammatory Disease, Rheumatoid Arthritis

Packaging

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H290,H312,H314,H317,H411
• Precautionary Statements: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - Skin Sens. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials