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MABS514

Sigma-Aldrich

Anti-Keap1 Antibody, clone 144

clone 144, from rat

Synonym(s):

Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

144, monoclonal

species reactivity

human, mouse

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KEAP1(9817)

General description

KEAP1 is an important adapter protein important in regulating the transcription factor NFE2L2/NRF2. Nuclear factor erythroid-2 related factor 2 (NRF2) is a redox-sensitive transcription factor that positively regulates the expression of genes encoding antioxidants, xenobiotic detoxification enzymes, and drug efflux pumps, and confers cytoprotection against oxidative stress and xenobiotics in normal cells. KEAP1 negatively regulates NRF2 activity by targeting it to proteasomal degradation. KEAP1 acts as a substrate adapter protein for the E3 ligase ubiquitin protein complex formed by CUL3 and RBX1 proteins which mediate the ubiquitination and proteasome destruction of NRF2. KEAP1 also targets PGAM5 which is a central mediator of programmed necrosis induced by TNF and reactive oxygen species, so again KEAP1 plays an important role ultimately in cell survival and adaptation to its microenvironment. KEAP1 is widely expressed in many tissues, most strongly in muscle tissues and appears to shuttle from the cytoplasm to the nucleus depending upon the cellular context.

Immunogen

Recombinant protein corresponding to mouse Keap1.

Application

Flow Cytometry Analysis: 4.0 µg of this antibody from a representative lot detected Keap1 in 1X10E6 NIH/3T3 cells.
Western Blotting Analysis: A representative lot detected Keap1 in extracts from mouse embreyonic fibroblasts (MEF′s) (Watai, Y., et al. (2007). Genes to Cells. 12:1163-1178).
Western Blotting Analysis: A representative lot detected Keap1 in transfected HeLa cells (Tsuchiya, Y., et al. (2011). Mole. and Cell Biol. 31(22):4500-4512).
Immunocytochemistry Analysis: A representative lot detected Keap1 in mouse embreyonic fibroblasts (MEF′s) cells (Watai, Y., et al. (2007). Genes to Cells. 12:1163-1178).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Anti-Keap1 Antibody, clone 144 is validated for use in Western Blotting and Flow Cytometry and Immunocytochemistry for the detection of Keap1.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Keap1 in 10 µg of Raji cell lysate.

Target description

~69 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chinmai Patibandla et al.
Redox biology, 71, 103117-103117 (2024-03-14)
Accumulation of reactive oxygen species (i.e., oxidative stress) is a leading cause of beta cell dysfunction and apoptosis in diabetes. NRF2 (NF-E2 p45-related factor-2) regulates the adaptation to oxidative stress, and its activity is negatively regulated by the redox-sensitive CUL3
Nikolaos Georgakopoulos et al.
Journal of medicinal chemistry, 65(10), 7380-7398 (2022-05-14)
Inhibitors of Kelch-like ECH-associated protein 1 (Keap1) increase the activity of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) by stalling its ubiquitination and degradation. This enhances the expression of genes encoding proteins involved in drug detoxification, redox
Dylan G Ryan et al.
iScience, 25(2), 103827-103827 (2022-02-25)
To overcome oxidative, inflammatory, and metabolic stress, cells have evolved cytoprotective protein networks controlled by nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) and its negative regulator, Kelch-like ECH associated protein 1 (Keap1). Here, using high-resolution mass spectrometry we characterize the
Sharadha Dayalan Naidu et al.
STAR protocols, 3(2), 101265-101265 (2022-04-09)
Pharmacologic inhibition of the protein-protein interaction (PPI) interface of the Keap1:Nrf2 complex, which leads to Nrf2 activation and cytoprotective gene expression, offers a promising strategy for disease prevention and treatment. To facilitate identification and validation of small-molecule Keap1:Nrf2 PPI inhibitors
Sharadha Dayalan Naidu et al.
iScience, 25(1), 103703-103703 (2022-01-18)
Transcription factor Nrf2 and its negative regulator Keap1 orchestrate a cytoprotective response against oxidative, metabolic, and inflammatory stress. Keap1 is a drug target, with several small molecules in drug development. Here, we show that the isoquinoline PRL-295 increased Keap1 thermostability

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