ZMS1028

Anti-α-Dystroglycan Antibody, clone VIA4-1 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Dystrophin-associated glycoprotein 1
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: VIA4-1, recombinant monoclonal, monoclonal
• Application: ICC, IHC (p), WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: VIA4-1, recombinant monoclonal, monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 97.44 kDa, observed mol wt ~140 kDa
• species reactivity: rabbit, human, mouse
• species reactivity (predicted by homology): guinea pig, canine, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q28685
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... DAG1(1605)

Description

General description

Dystroglycan (UniProt: Q28685; also known as Dystrophin-associated glycoprotein 1) is encoded by the DAG1 gene (Gene ID: 100009278) in rabbit. Dystroglycan is a component of the dystrophin glycoprotein complex that serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce alpha-dystroglycan (aa 30-653) and beta-dystroglycan (aa 654-895). Alpha-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin, agrin, neurexin, and pikachurin. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. Beta-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, beta-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, alpha/beta dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant alpha-dystroglycan glycosylation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sudo, A., et al. (2018). Human Mol. Genetics. 27(7); 7 1174-1185; Street, RM., et al. (2012). Reproductive Sci. 19(3); 282-289; Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Immunogen

Rabbit skeletal muscle membrane preparations.

Application

Anti-a-Dystroglycan, clone VIA4-1 ZooMAb, Cat. No. ZMS1028, is a recombinant Mouse monoclonal antibody that targets a-Dystroglycan and is tested for use in Immunocytochemistry, Immunohisttochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected -Dystroglycan in mouse placenta and human heart tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -Dystroglycan in HeLa and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected -Dystroglycan in rabbit skeletal muscle and human skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

CloneVIA4-1 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects alpha-Dystroglycan.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1