This product is stored at -20°C, however storing the powder form at -70°C is not expected to be detrimental to the material. Prepared stock solutions may be stored up to 2 months at -70°C, when aliquoted to plastic tubes or vials.
T6884
Thrombin from human plasma
lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)
Synonym(s):
Factor IIa
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
Specific activity:
≥2,000 NIH units/mg protein (E1%/280, 18.3)
Biological source:
human plasma
Pricing and availability is not currently available.
General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Thrombin is produced from the proteolytic cleavage of inactive prothrombin in the liver. It is a glycoprotein with four structural domains: the 10 γ-carboxylated glutamic acid containing Gal domain, two kringle domains, and the trypsin-like serine protease domain. The prothrombin gene is mapped to human chromosome 11p11.2.
Application
Thrombin from human plasma has been used:
- in the preparation of fibrin gels for assessing human bone marrow stromal cells (hBMSC) morphology,
- in the activation of platelets,
- to evaluate the integrity of endothelial cell (EC) monolayers
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags.
Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the protein C pathway in intestinal barrier function. [1]
The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles of the brain may contribute to the aggregation of tau protein and pathophysiology of Alzheimer disease.
Physical form
Lyophilized from saline sodium citrate buffer, pH 6.5
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information - |
| biological source human plasma | biological source - | biological source human plasma | biological source bovine plasma |
| specific activity ≥2,000 NIH units/mg protein (E1%/280, 18.3) | specific activity ≥1,000 NIH units/mg protein (E1%/280, 18.3) | specific activity - | specific activity 50 NIH units/mg dry wt |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 37.4 kDa | mol wt 37.4 kDa | mol wt - | mol wt - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is it possible to store Thrombin T6884 at -70°C, or only at -20°C?
1 answer-
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How do you have to reconstitute the product before using it? dH2O?
1 answer-
This product is soluble in water at 10 mg/mL. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/599/516/t6884pis.pdfHelpful?
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I want to reconstitute thrombin(T6884) and store it for long term storage at -70°C. I saw that it is stable if it contains 0.1% BSA when reconstituted, can I use PBS with 0.1% BSA?
1 answer-
The product information sheet recommends using water for reconstitution.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/599/516/t6884pis.pdfHelpful?
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does the thrombin in lotnr T6884 contaning his-tag, which can be removed by Ni affinity column after fusion protein cutting?
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This product is a native enzyme from human plasma and does not have a his-tag. Please see the link below to review the product datasheet for additional information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/599/516/t6884pis.pdf
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How do I convert 100 units of this thrombin into grams? For a given volume, to determine the concentration, I need to know the exact amount of thrombin in grams which I cannot find anywhere.
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The unit per milligram value is reported in the lot-specific Certificate of Analysis. This product has a minimum activity specification of 2000 units per milligram. The amount of powder per vial can be determined by dividing the package size by the unit/mg protein reported on the Certificate of Analysis.
See the link below to review a sample Certificate of Analysis. For this specific lot, the 100UN vial will contain 36.5ug and the 5KU vial will contain 1.83mg.
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T6884-100UNSLCP0517.pdfHelpful?
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What expiry should be applied to Thrombin when stored at -70C at 100UN/mL
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After reconstitution, thrombin is stable in water for about one week at 0-5°C. If aliquoted in plastic tubes stock solutions are stable for up to 2 months at -70°C.
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Can a stock solution of Product T6884, Thrombin, be stored frozen?
1 answer-
A suggested stock solution is 100 units/ml. The solution should contain 0.1% BSA for stability and may be stable for about one week at 0-5°C. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at -20°C or below.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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