This material is not reported as being light sensitive.
SML1046
CHIR99021
≥98% (HPLC), powder, GSK-3 inhibitor
Synonym(s):
6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile, CT99021
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About This Item
Empirical Formula (Hill Notation):
C22H18Cl2N8
CAS Number:
Molecular Weight:
465.34
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
MDL number:
Assay:
≥98% (HPLC)
Form:
powder
Pricing and availability is not currently available.
Product Name
CHIR99021, ≥98% (HPLC)
Quality Segment
assay
≥98% (HPLC)
form
powder
color
white to light brown
solubility
DMSO: 2 mg/mL, clear (warmed)
storage temp.
−20°C
SMILES string
ClC(C=C1Cl)=CC=C1C2=NC(NCCNC(N=C3)=CC=C3C#N)=NC=C2C4=NC(C)=CN4
InChI
1S/C22H18Cl2N8/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32)
InChI key
AQGNHMOJWBZFQQ-UHFFFAOYSA-N
General description
CHIR99021 is an aminopyrimidine derivative. CHIR99021 promotes self-renewal potential of embryonic stem cells (ESCs) of mice by inhibiting glycogen synthase kinase-3 (GSK-3) activity and potentiates the upregulation of β-catenin and c-Myc functions. CHIR99021 promotes self-renewal of ESCs by modulating transforming growth factor β (TGF-β), Notch and mitogen-activated protein kinases (MAPK) signaling pathways. CHIR99021 is an agonist of wingless/integrated (wnt) signalling, upregulates cyclinA expression and promotes cell proliferation in non-small-cell lung cancer (NSCLC) cell lines.
Application
CHIR99021 has been used as a Wnt signaling agonist to study its effects on the differentiation of human pluripotent stem cell-derived cardiomyocytes (hPSCs).[1][2]
CHIR99021 has been used:
- in cocktail with small molecules to convert human lung fibroblast cells into neurons
- as a culture medium supplement in potassium supplemented simplex optimization medium (KSOM) for the culture of blastocysts
- for chronic inhibition of GSK-3 to investigate its role in corticogenesis
Biochem/physiol Actions
CHIR99021 is a very potent inhibitor of Glycogen synthase kinase 3 (GSK3, IC50 values for GSK3α and GSK3β = 10 and 6.7 nM, respectively). There is no cross-reactivity against CDKs. CHIR99201 potentiates self-renewal of human and mouse embryonic stem cells, and enhances reprogramming of somatic cells into stem cells.
CHIR99021 is a very potent inhibitor of Glycogen synthase kinase 3.
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This Item | |||
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥97% (HPLC) |
| form powder | form powder | form powder | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility DMSO: 2 mg/mL, clear (warmed) | solubility DMSO: 0.5 mg/mL, clear (warmed) | solubility DMSO: >10 mg/mL | solubility DMSO: 5 mg/mL |
| color white to light brown | color white to brown | color white to beige | color light beige |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
No data available
flash_point_c
No data available
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Is CHIR99021 light sensitive?
1 answer-
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What is the solubility of Product SML1046?
1 answer-
The solubility of Product SML1046 has been tested in DMSO at 2 mg/ml when warmed. Although testing in water has not been conducted, it is expected to be less than 1 mg/ml at 25°C, similar to its solubility in saline. Additionally, solubility testing in ethanol has also shown a solubility of less than 1 mg/ml at 25°C.
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How is Product SML1046, CHIR99021, to be dissolved? And once dissolved, is the solution stable?
1 answer-
This product is soluble in DMSO (warmed) at 2-5 mg/mL. Solubility in polar solvents such as water, saline, or ethanol, is expected to be <1 mg/ml at 25° C. Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 6 months at -20°C. Avoid repeated freeze/thaw cycles and protect from light.
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