S2177

Anti-Synaptotagmin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD01092496
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 65 kDa
• species reactivity: rat
• technique(s): microarray: suitable; western blot: 1:5,000 using extract of rat brain membrane fraction
• UniProt accession no.: P21707
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SYT1(6857) ; mouse ... Syt1(20979) ; rat ... Syt1(25716)

Description

General description

Synaptotagmin (Syt, p65) is an abundant synaptic vesicle (SV) membrane protein. It is characterized by a short intravesicular N-terminal domain, a single transmembrane region and two copies of highly conserved internal repeats, known as the C2A and C2B domains, which are homologous to the C2 regulatory region of protein kinase C (PKC) in the cytoplasmic domain. At least eight different isoforms of synaptotagmin (SytI-VIII) are expressed in the brain, four of which (Syt IV, V, VII and VIII) are also expressed in non-neuronal tissues.

Specificity

The sequence is highly conserved among species (SytI) and is not found in other known synaptotagmin isoforms (SytII-VIII).

Immunogen

synthetic peptide corresponding to N-terminus of synaptotagmin I (SytI) of rat origin (amino acids 1-16 with C-terminally added lysine), conjugated to KLH.

Application

Anti-Synaptotagmin antibody produced in rabbit has been used:

• for cell surface labelling of synaptotagmin I (SytI)
• in immunofluorescence microscopy
• in western blotting

Biochem/physiol Actions

Synaptotagmin binds Ca²⁺ phospholipids with high affinity and has a central role in Ca²⁺ regulated neurotransmitter release. Synaptotagmin functions as a Ca²⁺ sensor and is required for efficient exocytosis, particularly in the vesicle docking and/or fusion step with the plasma membrane. Ca²⁺ influx triggers synaptotagmin to interact with either syntaxin or SNAP-25 and the cytoplasmic domain of neurexin leading to fusion and exocytosis. Mutations or deletion of synaptotagmin result in severely impaired Ca²⁺ triggered neurotransmitter release. Synapses of SytI knockout mice lack the fast-component of Ca²⁺ dependent neurotransmitter release, but exhibit no changes in the slow, Ca²⁺ independent component of synaptic vesicle exocytosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable