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RABHRP3

Sigma-Aldrich

HRP-Streptavidin

Synonym(s):

HRP streptavidin, HRP-conjugated streptavidin

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

shipped in

wet ice

storage temp.

−20°C

General description

Streptavidin is a biotin-binding protein. Horseradish peroxidase (HRP) is a heme-containing enzyme. HRP-Streptavidin consists of the protein streptavidin, as a secondary detection reagent, conjugated to the enzyme HRP. The conjugate is used in ELISA to increase the detection of various kinds of targets.

Application

HRP-Streptavidin has been conjugated to secondary antibodies for western blotting.

Other Notes

This item is for RAB series EIA kits.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jayendrakumar Patel et al.
Archives of virology, 165(3), 627-641 (2020-01-23)
HCV is a potential cause of viral hepatitis, which leads to blood-borne chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Anti-HCV antibody detection assays detect HCV infection after approximately 70 days. HCV core antigen can be detected much earlier than anti-HCV
Functional Protein Microarrays in Drug Discovery (2007)
Namjeong Choi et al.
Cells, 9(12) (2020-12-16)
Splicing factor 3b subunit 1 (SF3B1) is an essential protein in spliceosomes and mutated frequently in many cancers. While roles of SF3B1 in single intron splicing and roles of its cancer-linked mutant in aberrant splicing have been identified to some
Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay
Lakshmipriya T, et al.
PLoS ONE, 11(3), e0151153-e0151153 (2016)
Zhen Wang et al.
Electrophoresis, 42(6), 793-799 (2020-12-24)
Western blot (protein immunoblot) is a widely used analytical technique in molecular biology. Utilizing the specific recognizing primary antibody, proteins immobilized on various matrix are investigated by subsequent visualization steps, for example, by the horse radish peroxidase conjugated secondary antibody

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