A separate assay would be required to determine the total milligrams of protein per liter. The result of ((U Glo-1 / L) / (mg protein / L)) provides the value of U Glo-1 per milligram of protein.
MAK114
Glyoxalase I Activity Assay Kit
sufficient for 100 tests (UV)
Synonym(s):
Aldoketomutase Activity Assay, GLO1 Activity Assay, Ketone-Aldehyde Mutase, Activity Assa, Lactoylglutathione Lyase Activity Assay, Methylglyoxalase Activity Assay
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Pricing and availability is not currently available.
usage
sufficient for 100 tests (UV)
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
human ... GLO1(2739)
mouse ... GLO1(109801)
rat ... GLO1(294320)
General description
Glyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans, and cancer.
Application
Suitable for the detection of Glyoxalase I activity in enzyme preparations and biological samples. Suitable for testing the effects of compounds on Glyoxalase I activity.
Glyoxalase I Assay Kit has been used to estimate the glyoxalase I activity in plasma samples.
Biochem/physiol Actions
The Glyoxalase I Activity Assay kit provides a simple and direct procedure for measuring GLO-1 activity in a variety of samples such as enzyme preparations or biological samples. In this assay, the GLO-1-mediated production of S-lactoylglutathione is measured by monitoring the change in absorbance at 240 nm. One unit of Glyoxalase I is the amount of enzyme that will convert 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.6 and 25 °C.
Features and Benefits
Compatible with high-throughput handling systems.
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This Item | ||
|---|---|---|
| description sufficient for 100 tests (UV) | description sufficient for 100 colorimetric or fluorometric tests | description sufficient for 100 colorimetric or fluorometric tests |
| Gene Information human ... GLO1(2739) | Gene Information - | Gene Information - |
| detection method colorimetric | detection method fluorometric, colorimetric | detection method fluorometric, colorimetric |
| usage sufficient for 100 tests (UV) | usage - | usage sufficient for 100 colorimetric or fluorometric tests |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) cancer | relevant disease(s) endocrinological disorders, diabetes | relevant disease(s) drug-induced liver injury; non-alcoholic/simple fatty liver (NAFL); cancer |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
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How to normalize the results using MAK114 Glyoxalase I Activity Assay with protein amount (units/mg(protein))?
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