This product concentration specification of 9.5-11.5 mg/mL. The unit activity is not determined. However, based on the starting material, an activity range of 28 -29 IU/mg is expected. Therefore, the product would have approximately 280,000 units per liter volume. This is a recombinant human insulin prepared in a 25 mM HEPES buffer. The solution may be diluted with an aqueous medium or buffer of choice.
I9278
Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture
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About This Item
Form:
solution
Pricing and availability is not currently available.
General description
Insulin is a two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond.
Application
Insulin solution human is recommended for use in cell culture applications at 0.5 to 1mL per liter of medium.
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).
Insulin solution human has been used as a supplement in Dulbecco′s modified eagle′s medium (DMEM) to culture epithelial cell line and human endometrial cell line, minimum essential medium eagle to culture human breast adenocarcinoma cell line and medium 199 to culture normal ovarian cell line.
Biochem/physiol Actions
Insulin plays a role in various cellular processes, such as promoting cell growth, regulating the cell cycle, and facilitating glucose uptake. It is commonly used as a protein supplement in serum-free mammalian cell culture media to help promote the optimal growth and maintenance of mammalian cells.
Physical form
stable 2-8 °C
Other Notes
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
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This Item | |||
|---|---|---|---|
| description sterile-filtered, BioXtra, suitable for cell culture | description meets USP testing specifications | description recombinant, expressed in yeast, γ-irradiated, suitable for cell culture | description European Pharmacopoeia (EP) Reference Standard |
| product line BioXtra | product line - | product line - | product line - |
| Quality Level 400 | Quality Level 200 | Quality Level 200 | Quality Level - |
| form solution | form solid | form lyophilized powder | form - |
| concentration 9.5-11.5 mg/mL | concentration - | concentration - | concentration - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| pH 7.9-8.5 | pH - | pH - | pH - |
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Cuantas UI/L tiene este preparado de insulina? Es insulina humana recombinante En que se puede diluir
1 answer-
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How can I know the exact concentration of my product? I know the lot number
1 answer-
The concentration of this product may range from 9.5–11.5 mg/mL. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i9278#product-documentationHelpful?
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some vendors provide insulin solution in low ph， how come this one in neutral ph
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This product is formulated at a neutral to slightly alkaline pH using a HEPES buffer (pH 7.9–8.5) to enhance long-term stability and solubility during storage. This approach differs from some vendors who use a low pH formulation for immediate solubilization.
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In which solution was insulin dissolved?
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This is in HEPES. 10mg/mL concentration in 25 mM Hepes
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What's the half-life of this insulin within the media at 37C?
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Unfortunately, the half-life of this insulin within the media at 37 °C has not been tested.
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Can I aliquot the insulin and freeze it? Is it still functional after thawing?
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This product is recommended to be stored at 2-8°C. For research applications, freezing insulin solution is a common practice. However, solutions available on the commercial market for therapeutic use are not frozen to maintain maximum activity. No stability studies have been performed on the shelf life of frozen insulin solutions.
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What is the concentration of this solution in (Units/mL) i.e. if my stock is at a concentration of 11.1 mg/mL, what is that in Units/mL?
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The specification for the product does not include units of potency.
However, the potency is typically about 28 -29 IU/mg.Helpful?
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What is the source of this insulin and is the solutuion expected to also contain proinsulin?
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This product is a biosynthetic product, produced by recombinant expression in yeast (Saccharomyces cerevisiae), with a synthetic gene insert encoding human insulin precursor. The proinsulin content is not among the testing specifications for this product.
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How is this insulin produced? In E.Coli? Yeast?...?
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This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.
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Is this product suitable for insulin tolerance tests in mice
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This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/Helpful?
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