I1896

Interleukin-13 from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-13
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00675503

Properties

• biological source: mouse
• Quality Segment: 100
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1-10 ng/mL EC₅₀
• mol wt: 11.5 kDa (predicted; 9 kDa under reducing conditions)
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P20109
• storage temp.: −20°C
• Gene Information: mouse ... Il13(16163)

Description

Biochem/physiol Actions

Interleukin-13 is a pleiotropic cytokine produced by activated Th2 cells in the mouse and human. IL-13 is secreted mainly as an unglycosylated protein of 132 amino acids with a molecular weight of 10,000 daltons. A 4.3kb DNA fragment of the mouse IL-13 gene was sequenced and occurs as a single copy mapping a chromosome 11. In the human, a 4.6 kb DNA segment of the IL-13 gene occurs as a single copy and maps to chromosome 5. IL-13 induces B cell proliferation and also induces IgE switching. The proliferative activity of human and mouse IL-13 products are tested in culture using the human erythroleukemic cell line TF-1.

Physical form

lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS), pH 7.4, with 50 μg bovine serum albumin (BSA) per 1 μg of cytokine

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves