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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
H6269
Hexadecyltrimethylammonium bromide
Molecular Biology, ≥99%
Synonym(s):
CTAB, Cetrimonium bromide, Cetyltrimethylammonium bromide, Palmityltrimethylammonium bromide
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About This Item
grade
Molecular Biology
Quality Segment
description
cationic
assay
≥99%
form
powder or crystals
mol wt
micellar avg mol wt 62,000
aggregation number
170
pH
5.0-7 (25 °C, 36.4 g/L)
CMC
0.92, 1 mM (20-25°C)
mp
248-251 °C (lit.)
solubility
H2O: 1/10 g/mL
HLB
10
suitability
suitable for DNA precipitation
foreign activity
DNAse (Detection), NICKase Detection , RNAse, none detected
SMILES string
[Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C
InChI
1S/C19H42N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(2,3)4;/h5-19H2,1-4H3;1H/q+1;/p-1
InChI key
LZZYPRNAOMGNLH-UHFFFAOYSA-M
Application
- as a component of DNA extraction buffer and RNA extraction buffer
- as a constituent of dye dissolution buffer
Biochem/physiol Actions
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for DNA precipitation | suitability - | suitability - | suitability corresponds to standard for filter test |
| form powder or crystals | form crystalline, powder or crystals | form powder or chunks | form powder or crystals |
| grade Molecular Biology | grade - | grade - | grade - |
| assay ≥99% | assay ≥99% | assay ≥96.0% (AT) | assay ≥99.0% (AT) |
| aggregation number 170 | aggregation number 170 | aggregation number - | aggregation number 170 |
| mp 248-251 °C (lit.) | mp 248-251 °C (lit.) | mp 248-251 °C (lit.) | mp 248-251 °C (lit.) |
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signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral - STOT SE 3
target_organs
Gastrointestinal tract, Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
471.2 °F - closed cup
flash_point_c
244 °C - closed cup
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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수용액에 대한 용해도가 36.4g/Liter (100 mM)로 되어 있는데, 1% (29 mM) 수용액을 제작하여 Light Scattering으로 Particle를 측정 시 0.5 um 크기 이상의 particle이 5000 개 /mL이상 측정됩니다. CMC(0.9 mM) 보다 농도가 높아 Micelle이 형성되었나 하는 생각도 있지만, Micelle 대비 입자 크기가 커서 무엇인지 궁금합니다.
1 answer-
For Product H6269 (commonly known as CTAB), the micellar properties have been well studied. The formation of the micelles is dependent on the pH of the solution, as well as the presence of any additives. The CMC has been reported variously as either 0.9 mM or 1.0 mM, so at 29 mM a great majority of the CTAB should be in the form of micelles. The large particles being observed may be aggregates of micelles.
Running a control sample at a lower CTAB concentration (around 1 mM) would be recommended.
The micellar properties have been studied by both static light scattering and dynamic light scattering; see:
https://www.sciencedirect.com/science/article/pii/S0927775714003835#bib0030
The second paragraph of the Introduction section of that paper cites references 12, 4 and 5.Helpful?
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