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F6140

Sigma-Aldrich

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

clone FN-3E2, ascites fluid

Synonym(s):

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FN-3E2, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

mouse, human, chicken

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:400 using cultured chicken fibroblasts
radioimmunoassay: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

General description

Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.

Specificity

The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.

Immunogen

fibronectin antigen released in culture by a human breast cancer cell line

Application

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:
  • immunoblot
  • immunofluorescent microscopy
  • immunocytochemistry

Biochem/physiol Actions

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .
Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fibronectin in cell adhesion and migration via N-glycosylation
Hsiao CT, et al.
Oncotarget, 8(41), 70653-70653 (2017)
Critical role of the cAMP-PKA pathway in hyperglycemia-induced epigenetic activation of fibrogenic program in the kidney
Deb DK, et al.
Faseb Journal, 31 (2017)
S Stenman et al.
Acta medica Scandinavica. Supplementum, 642, 165-170 (1980-01-01)
Fibronectin is a polymorphic glycoprotein of plasma, other body fluids and connective tissue, and it occurs in an insoluble and a soluble form. Insoluble fibronectin is found associated with basement membranes and in loose connective tissue matrix as well as
Lisandra E de Castro Brás et al.
Journal of proteomics, 86, 43-52 (2013-05-18)
The extracellular matrix (ECM) is a critical tissue component, providing structural support as well as important regulatory signaling cues to govern cellular growth, metabolism, and differentiation. The study of ECM proteins, however, is hampered by the low solubility of ECM
Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment
McLane JS, et sl.
Biophysical Journal, 109 (2015)

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