OE21 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96062201, human esophagus, Epithelial

• Synonym(s): JROECL 21 Cells, OE 21 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human esophagus
• packaging: tube of 5 μg 96062201-DNA-5UG; pkg of vial of cells 96062201-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Epithelial
• products: Not specified
• receptors: MHC class I
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian oesophageal squamous cell carcinoma

Cell Line Description

OE21, also known as JROECL21, was established in 1993 from a squamous carcinoma of mid oesophagus of a 74 year-old male patient. The tumour was identified as pathological stage IIA(UICC) and showed moderate differentiation. HLA-A,-B and -C (MHC class I) are expressed constitutively, expression of ICAM-1 can be induced by treatment with interferon-gamma. OE21 cells express epithelial cytokeratins and are tumourigenic in nude mice.

Application

Study of oesophageal cancer

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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