24365C
EX-CELL® CD CHO Fusion
chemically defined cell culture medium, animal-component free, without L-glutamine, without hypoxanthine, without sodium bicarbonate, without thymidine
Synonym(s):
CHO Cell Culture Medium, CHO Suspension Culture Medium, Chemically Defined CHO Medium, GS Selection Medium, L-Glutamine-Free CHO Medium, Mammalian Cell Culture Medium, Recombinant Protein Production Medium
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About This Item
Product Name
EX-CELL® CD CHO Fusion, Chemically Defined Medium for Long-Term CHO Cell Culture, without L-Glutamine
Quality Segment
biological source
non-animal source
description
chemically defined cell culture medium, Animal-component free, without L-glutamine, without hypoxanthine, without sodium bicarbonate, without thymidine.
product line
EX-CELL®
form
dry powder
technique(s)
cell culture | mammalian: suitable (suspension)
application(s)
cell culture
media preparation
pharma/biopharma processes
compatibility
for use with CHOZN® and CHO Cell Lines
components
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
General description
Application
- Long-Term CHO Cell Culture (Suspension) -Supports ≥95% viability and ≥2×10⁶ cells/mL for robust, reproducible CHO suspension culture
- GS Selection Workflows - Suitable for CHO cells using glutamine synthetase (GS) selection, including the CHOZN® GS knockout cell line; supplied without L-glutamine to support GS-based selection systems
- DHFR⁻ Gene Amplification including the CHOZN® DHFR knockout cell line
- Monoclonal Antibody (mAb) Development & Manufacturing - Supports CHO-based production of secreted recombinant proteins and antibodies, as demonstrated using rIgG-producing CHO cell lines
Features and Benefits
- Chemically Defined Medium: Supports consistent andreproducible cell culture performance.
- Animal Component-Free: Reduces variability associated withanimal-derived components.
- Optimized for CHO Cells: Supports long-term growth andmaintenance of CHO cells.
- L-Glutamine Free: Enhances media stability and reducesammonia accumulation.
- Hypoxanthine-Free: Suitable for DHFR-based cell linedevelopment workflows.
- Thymidine-Free: Enables use in gene amplificationapplications.
- Macromolecule-Free Formulation: Facilitates isolation andpurification of secreted proteins.
- Suspension Culture Compatible: Supports scalable cellculture processes.
- Supports GS Selection Systems: Suitable for glutaminesynthetase-based selection workflows
Preparation Note
Legal Information
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| description Chemically Defined Medium for Long-Term CHO Cell Culture, without L-Glutamine | description Serum-Free Medium for CHO cells, Chemically Defined Animal-component free, without L-glutamine, without hypoxanthine, without sodium bicarbonate, without thymidine, dry powder, suitable for cell culture. Use 19.24 g/L. See information sheet, 24360C @ www.sigmaaldrich.com for hydration instructions | description CHO Feed 1 (with glucose). Use 64.10 g/L. See information sheet, 24367C @ www.sigmaaldrich.com for hydration instructions | description Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture |
| application(s) cell culture | application(s) - | application(s) - | application(s) - |
| technique(s) cell culture | mammalian: suitable (suspension) | technique(s) cell culture | mammalian: suitable (suspension) | technique(s) cell culture | mammalian: suitable (suspension) | technique(s) cell culture | mammalian: suitable (suspension) |
| form dry powder | form dry powder | form dry powder | form liquid |
| components L-glutamine: no | components - | components - | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| product line EX-CELL® | product line EX-CELL® | product line EX-CELL® | product line EX-CELL® |
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Storage Class
13 - Non Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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