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Sigma-Aldrich

TRIS Glycine buffer solution

BioUltra, 10× concentrate

Synonym(s):

Blotting buffer solution

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

product line

BioUltra

Quality Level

100

form

liquid

quality

10× concentrate

composition

glycine, 1.92 M
TRIS, 0.25 M

impurities

insoluble matter, passes filter test
 proteases, none detected

ign. residue

≤0.2%

pH

8.5-8.7

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
NH4+: ≤200 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.1

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Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Other Notes

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6832
BCCJ1880
BCCG8307
BCCD4254
BCCC3019

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E R Frears et al.
Glycoconjugate journal, 16(6), 283-290 (1999-12-01)
IgG carries bi-antennary N-linked glycans which differ in degrees of galactosylation, core fucosylation and bisecting N-acetyl glucosamine. The majority of these are non-sialyated closely related neutral structures which can be resolved by HPLC analysis, but which are difficult to separate
Hongshan Liu et al.
Molecular vision, 15, 505-517 (2009-03-06)
To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde
Guo-Min Tan et al.
Journal of chromatography. A, 1098(1-2), 131-137 (2005-11-30)
Ion-exchange electrochromatography with an oscillatory electric field perpendicular to mobile-phase flow driven by pressure (pIEEC) was developed with a column design of rectangle cross-section. The effect of electric field strength on the dynamic binding capacity (DBC) was examined by frontal
Zhixin Wang et al.
Electrophoresis, 29(22), 4454-4462 (2008-11-28)
Multiple labeling of nucleic acids by intercalative dyes is a promising method for ultrasensitive nucleic acid assays. The properties of the fast dissociation and instability of dye-DNA complexes may prevent from their wide applications in CE-LIF nucleic acid analysis. Here
H Towbin et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4350-4354 (1979-09-01)
A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was

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