RNAINH-RO
Roche
Protector RNase Inhibitor
Synonym(s):
protector, rnase inhibitor
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
41181611
NACRES:
NA.21
Recommended Products
Assay
>95% (SDS-PAGE)
Quality Level
mol wt
~50 kDa
packaging
pkg of 10,000 U (03335402001 [5 x 2,000 U])
pkg of 2,000 U (03335399001)
manufacturer/tradename
Roche
parameter
25-55 °C optimum reaction temp.
pH range
5.0-9.0
storage temp.
−20°C
Related Categories
General description
Contents
Protector RNase Inhibitor (40 U/μl) in storage buffer: 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol (v/v), pH approximately 7.6 (+4°C).
Protector RNase Inhibitor (40 U/μl) in storage buffer: 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol (v/v), pH approximately 7.6 (+4°C).
Protector RNase Inhibitor inactivates a wide spectrum of RNases, including
- RNase A
- RNase B
- RNase T2
- protect mRNA during cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), or in vitro transcription/translation reactions
- protect viral RNA during in vitro virus replication
- inhibit RNases during RNA isolation and purification
- be used in RNase protection assays
- help prepare RNase-free antibodies
Specificity
Protector RNase Inhibitor definetely does not inhibit RNase T1 and RNase 1.
Protector RNase Inhibitor (20 U/ 20 μl reaction) inhibits RNase A up to 1 ng, RNase B up to 160 pg and RNase T2 up to 0.03 U/μl reaction volume.
RNase H is not inhibited by Protector RNase Inhibitor.
Heat inactivation: > 65 °C
Protector RNase Inhibitor (20 U/ 20 μl reaction) inhibits RNase A up to 1 ng, RNase B up to 160 pg and RNase T2 up to 0.03 U/μl reaction volume.
RNase H is not inhibited by Protector RNase Inhibitor.
Heat inactivation: > 65 °C
Application
Protector RNase Inhibitor may be used to:
- Protect mRNA in cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), e.g., with the LightCycler® Instruments, in vitro transcription/translation system, RNase Protection Assay, in vitro RNA synthesis
- Protect viral RNA during in vitro virus replication
- Inhibit RNases during RNA isolation and purification
- Help prepare RNase-free antibodies
- Synthesize RNA probes for in situ hybridization
Features and Benefits
- Rely on a thermostable RNase inhibitor: Protector RNase Inhibitor remains stable even when using thermostable reverse transcriptases such as Transcriptor Reverse Transcriptase.
- Benefit from a wide range of reaction conditions: Protector RNase Inhibitor is active at pH 5.0 to 9.0 and at temperatures between +25°C to +55°C (partial activity is still measureable at +60°C).
- Eliminate interference in different RNA analysis applications: Maintain performance even when adding at 16-fold higher than the standard concentration.
- Insist on a highly-purified preparation: Each batch is function tested using techniques such as quantitative RT-PCR to ensure the absence of endonucleases, ribonucleases, or nicking activity, according to the current quality control procedures.
Quality
Each lot of Protector RNase Inhibitor is function tested with the Titan One Tube RT-PCR Kit and the LightCycler DPD Kit. Protector RNase Inhibitor is also tested for contaminating activities as described below.
Test buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.1 mM EDTA, 7 mM β-Mercaptoethanol; pH 7.5 (+37°C).
Absence of endonucleases: 1 μg EcoR I/Hind III*-fragments of λDNA is incubated with Protector RNase Inhibitor in 50 μl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no alteration in the banding pattern is stated under "Endo".
Absence of nicking activity: 1 μg supercoiled pBR322 DNA is incubated with Protector RNase Inhibitor in 50 μl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no relaxation of supercoiled DNA is stated under "Nick. Act.".
Absence of ribonuclease (1): 5 μg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C in a final volume of 50 μl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under "RNase 1".
Absence of ribonuclease (2): 5 μg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C, then 10 minutes at +65°C in a final volume of 50 μl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under "RNase 2".
Test buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.1 mM EDTA, 7 mM β-Mercaptoethanol; pH 7.5 (+37°C).
Absence of endonucleases: 1 μg EcoR I/Hind III*-fragments of λDNA is incubated with Protector RNase Inhibitor in 50 μl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no alteration in the banding pattern is stated under "Endo".
Absence of nicking activity: 1 μg supercoiled pBR322 DNA is incubated with Protector RNase Inhibitor in 50 μl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no relaxation of supercoiled DNA is stated under "Nick. Act.".
Absence of ribonuclease (1): 5 μg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C in a final volume of 50 μl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under "RNase 1".
Absence of ribonuclease (2): 5 μg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C, then 10 minutes at +65°C in a final volume of 50 μl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under "RNase 2".
Unit Definition
One unit of Protector RNase Inhibitor is defined as the amount of protein required to inhibit 50% of the activity of 5 ng RNase A.
Unit Assay: Activity is measured according to Blackburn as ability to inhibit hydrolysis of cyclic cytidine-2′ : 3′-monophosphoric acid. Under assay conditions, 200 U of Protector RNase Inhibitor inhibits 50% of the activity of 1 μg RNase A.
Volume Activity: 40 U/μl
Unit Assay: Activity is measured according to Blackburn as ability to inhibit hydrolysis of cyclic cytidine-2′ : 3′-monophosphoric acid. Under assay conditions, 200 U of Protector RNase Inhibitor inhibits 50% of the activity of 1 μg RNase A.
Volume Activity: 40 U/μl
Preparation Note
Working concentration:
of inhibitor did not interfere with RT-PCR.)
- 5 to 10 U in One-step RT-PCR
- 25 to 50 U in Two step RT-PCR
- 20 U for in vitro transcription
of inhibitor did not interfere with RT-PCR.)
Other Notes
Product Specifications:
Source: Rat lung; Product is produced recombinant in E. coli
Isoelectric Point: pH 4.5
Temperature where Protector RNase Inhibitor is active: 25°C to +55°C; partial activity is still measurable at +60°C
Inactivation: Under severe denaturizing conditions (temperatures above +65°C), the inhibitor is inactivated
Bioburden: <50 cfu/ml
DNA Content: <100 pg/mg
Source: Rat lung; Product is produced recombinant in E. coli
Isoelectric Point: pH 4.5
Temperature where Protector RNase Inhibitor is active: 25°C to +55°C; partial activity is still measurable at +60°C
Inactivation: Under severe denaturizing conditions (temperatures above +65°C), the inhibitor is inactivated
Bioburden: <50 cfu/ml
DNA Content: <100 pg/mg
For life science research only. Not for use in diagnostic procedures.
Legal Information
NOTICE TO PURCHASER: DISCLAIMER OF LICENSE
This product is optimized for use in the polymerase chain reaction (PCR) covered by patents owned by F. Hoffmann-La Roche Ltd ("Roche"). No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of this product. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Roche, Applied Biosystems or other licensed suppliers′ reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. Diagnostic purposes require a license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
This product is optimized for use in the polymerase chain reaction (PCR) covered by patents owned by F. Hoffmann-La Roche Ltd ("Roche"). No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of this product. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Roche, Applied Biosystems or other licensed suppliers′ reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. Diagnostic purposes require a license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
LightCycler is a registered trademark of Roche
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Monica Frinchi et al.
Acta histochemica, 115(3), 252-256 (2012-08-18)
Although connexin36 (Cx36) has been studied in several tissues, it is notable that no data are available on Cx36 expression in the carotid body and the intestine. The present study was undertaken to evaluate using immunohistochemistry, PCR and Western blotting
Jurgen Schnermann et al.
American journal of physiology. Renal physiology, 305(9), F1352-F1364 (2013-09-21)
Deletions of claudin-2 (Cldn2) and aquaporin1 (AQP1) reduce proximal fluid reabsorption (PFR) by about 30% and 50%, respectively. Experiments were done to replicate these observations and to determine in AQP1/claudin-2 double knockout mice (DKO) if the effects of deletions of
Stefanie Dukowic-Schulze et al.
Frontiers in plant science, 5, 57-57 (2014-03-13)
High-throughput sequencing has become the large-scale approach of choice to study global gene expression and the distribution of specific chromatin marks and features. However, the limited availability of large amounts of purified cells made it very challenging to apply sequencing-based
Beáta Szabó et al.
International journal of molecular sciences, 19(11) (2018-11-08)
Long non-coding RNAs (lncRNAs) are emerging as important regulators of cellular processes and are extensively involved in the development of different cancers; including leukemias. As one of the accepted methods of lncRNA function is affecting chromatin structure; lncRNA binding has
Adam D Langenbacher et al.
EvoDevo, 7, 9-9 (2016-04-14)
Germ cells are specified during early development and are responsible for generating gametes in the adult. After germ cells are specified, they typically migrate to a particular niche in the organism where they reside for the remainder of its lifetime.
Protocols
Protector RNase Inhibitor stabilizes RNA at -80°C without permeating intact cells, unsuitable for cell pellet addition.
Protector RNase Inhibitor stabilizes RNA at -80°C without permeating intact cells, unsuitable for cell pellet addition.
Protector RNase Inhibitor stabilizes RNA at -80°C without permeating intact cells, unsuitable for cell pellet addition.
Protector RNase Inhibitor stabilizes RNA at -80°C without permeating intact cells, unsuitable for cell pellet addition.
Related Content
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service