S7108

ApopTag Stop/Wash Buffer

ApopTag Stop/Wash Buffer is intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection.

• UNSPSC Code: 12161503
• NACRES: NA.32
• eCl@ss: 32161000

Properties

• Quality Segment: 100
• manufacturer/tradename: ApopTag, Chemicon^®
• technique(s): activity assay: suitable (apoptosis)
• shipped in: dry ice

Description

General description

ApopTag Stop/Wash Buffer is intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag In Situ Apoptosis Detection Kits (Catalog Numbers: S7100, S7101, S7111 and S7165).

Materials Provided

Each bottle contains 20 ml of ApopTag Stop/Wash Buffer.



ApopTag is a registered trademark of Serologicals Company.

Application

Instructions for Use

Prepare Working Strength Stop/Wash Buffer by adding:

1 ml ApopTag Stop/Wash Buffer

34 ml dH2O

35 ml Total



This amount is sufficient to treat 5 slides in a coplin jar. Working Strength Stop/Wash Buffer may be prepared in advance and stored in a glass or plastic container at 4°C for up to one (1) year. Use a fresh aliquot for each experiment.



Working Strength Stop/Wash Buffer is ready for use at the "APPLY STOP/WASH BUFFER" protocol step in The Complete ApopTag Manual.



NOTE: Agitate for 15 seconds and incubate for 10 min. at room temperature to avoid precipitation.

Preparation Note

Recommended Storage is -15°C to -25°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08,GHS05
• signalword: Danger
• hcodes: H290,H314,H373
• pcodes: P234 - P260 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P314
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk: WGK 2