MABS1245
Anti-Thrombomodulin Antibody, clone 273-34A
clone 273-34A, from rat
Synonym(s):
TM, Thrombomodulin, Fetomodulin, CD141
About This Item
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
273-34A, monoclonal
species reactivity
mouse, human
technique(s)
ELISA: suitable
electron microscopy: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
General description
Immunogen
Application
Immunoprecipitation Analysis: Representative lots immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblasts (NMLF), but not Line 1 mouse lung carcinoma cells (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
Immunohistochemistry Analysis: A representative lot detected upregulated thrombomodulin immunoreactivity associated with the neoepidermis during wound healing using formalin-fixed, paraffin-embedded mouse skin sections (Peterson, J.J., et al. (1999). Am. J. Pathol. 155(5): 1569–1575).
Immunohistochemistry Analysis: Representative lots detected epithelium thrombomodulin immunoreactivity in paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
Immunohistochemistry Analysis: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by fluorescent immunohistochemistry using frozen mouse lung sections (Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124)
ELISA Analysis: Representative lots were used as the capture antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 411-201B (Cat. No. MABS1273) as the detection antibody, followed by an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay using 125I-labeled clone 411-201B as the detection antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).
Electron Microscopy Analysis: Representative lots detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
Western Blotting Analysis: A representative lot detected purified thrombomodulin (P112) from normal mouse lung fibroblasts (NMLF) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Signaling
Signaling Neuroscience
Quality
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Thrombomodulin in mouse heart tissue.
Target description
Physical form
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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