Skip to Content
Merck
All Photos(1)

Key Documents

MAB4400

Sigma-Aldrich

Anti-Renilla Luciferase Antibody, clone 5B11.2

ascites fluid, clone 5B11.2, Chemicon®

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5B11.2, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

General description

Renilla luciferase is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Renilla luciferase is often used as a reporter gene in sudies of gene expression or interaction. The 36 kDa enzyme catalyzes oxidation of the substrate coelenterazine to coelenteramide, resulting in the production of visible light. Renilla luciferase is not the same as luciferase from fireflies (Photalis pyralis) nor does it recognize the same substrates, so both enzymes can be used simultaneously in the same assay.

Specificity

Renilla luciferase

Immunogen

Renilla luciferase GST fusion protein

Application

Detect Renilla Luciferase using this Anti-Renilla Luciferase Antibody, clone 5B11.2 validated for use in ELISA, FUNC, NEUT & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot:
A previous lot of this antibody was used in WB.

EIA:
A previous lot of this antibody was used in ELISA.

Neutralization:
A prevous lot of this antibody was used in NEAT.

Optimal working dilutions must be determined by end user.

Target description

36 kDa

Physical form

Mouse monoclonal IgG1 Ascites.
Unpurified

Storage and Stability

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Renilla

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Maija Pollari et al.
PLoS pathogens, 16(10), e1008965-e1008965 (2020-10-09)
In this study, we demonstrate a novel pro-viral role for the Nicotiana benthamiana ARGONAUTE 1 (AGO1) in potyvirus infection. AGO1 strongly enhanced potato virus A (PVA) particle production and benefited the infection when supplied in excess. We subsequently identified the
Jaume Lillo et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Cannabinoids have been reported as orexigenic, i.e., as promoting food intake that, among others, is controlled by the so-called "hunger" hormone, ghrelin. The aim of this paper was to look for functional and/or molecular interactions between ghrelin GHSR1a and cannabinoid
Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC.
Edmonds, TG; Ding, H; Yuan, X; Wei, Q; Smith, KS; Conway, JA; Wieczorek, L; Brown et al.
Virology null
Irene Reyes-Resina et al.
Biochemical pharmacology, 157, 169-179 (2018-06-06)
GPR18, still considered an orphan receptor, may respond to endocannabinoids, whose canonical receptors are CB1 and CB2. GPR18 and CB2 receptors share a role in peripheral immune response regulation and are co-expressed in microglia, which are immunocompetent cells in the
Inhibitor of MYC identified in a Krohnke pyridine library.
Hart, JR; Garner, AL; Yu, J; Ito, Y; Sun, M; Ueno, L; Rhee, JK; Baksh, MM; Stefan et al.
Proceedings of the National Academy of Sciences of the USA null

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service