HGAMMAG-301K

MILLIPLEX^® Human Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.

• Synonym(s): Human Ig isotype multiplex panel, Human immune globulin multiplex panel, Human immunoglobulin multiplex kit, Luminex^® human immunoglobulin immunoassay, Millipore Human immunoglobulin panel
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 88-99%; sensitivity: 0.08 ng/mL; (IgG₄); sensitivity: 0.09 ng/mL; (IgG₃); sensitivity: 0.47 ng/mL; (IgM); sensitivity: 1.5 ng/mL; (IgA); sensitivity: 10 ng/mL; (IgG₁); sensitivity: 85 ng/mL; (IgG₂); standard curve range: 0.2-150 ng/mL; (IgG₃); standard curve range: 0.4-300 ng/mL; (IgG₄); standard curve range: 14-10,000 ng/mL; (IgG₁); standard curve range: 2.1-1,500 ng/mL; (IgA); standard curve range: 3.4-2,500 ng/mL; (IgM); standard curve range: 41-30,000 ng/mL; (IgG₂)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Five major isotypes have been identified in placental mammals: IgM, IgG, IgA, IgE and IgD (B-cell receptor) – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. In addition, isotyping applications include analyzing hybridomas during antibody generation and vaccine development.

The MILLIPLEX^® Human Isotyping Bead Panel, measuring human IgG subclasses (1, 2, 3, 4), IgM and IgA is designed to enable you to measure both classes and subclasses accurately – all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Application

• Analytes: IgG1, IgG2, IgG3, IgG4, IgA, IgM
• Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant samples
• Recommended Sample dilution: 1:16,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1μg/mL Ig per well
• Assay Run Time: One day
• Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Components

MILLIPLEX MAP Anti-Human κ and λ Light Chain, PE

MILLIPLEX MAP Human Multi-Immunoglobulin Standard

MILLIPLEX MAP Human Immunoglobulin Positive Control

MILLIPLEX MAP Assay Buffer

MILLIPLEX MAP Wash Buffer, 10X

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H312 + H332,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 10 - Combustible liquids