189490
3-MA
≥95% (HPLC), powder, autophagy inhibitor, Calbiochem®
Synonym(s):
Autophagy Inhibitor, 3-MA, 3-Methyladenine
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Empirical Formula (Hill Notation):
C6H7N5
CAS Number:
Molecular Weight:
149.15
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
![[Ala1,3,11,15]-Endothelin 1](/deepweb/assets/sigmaaldrich/product/structures/257/425/5e642291-a643-4523-a7f1-65d3e6e51aa8/640/5e642291-a643-4523-a7f1-65d3e6e51aa8.png)
product name
Autophagy Inhibitor, 3-MA, Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker. Acts as an inhibitor of III PI3-Kinase.
Quality Level
Assay
≥95% (HPLC)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
white to faint yellow
solubility
DMSO: 10 mg/mL
water: 5 mg/mL
shipped in
ambient
storage temp.
2-8°C
InChI
1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
InChI key
FSASIHFSFGAIJM-UHFFFAOYSA-N
General description
A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Preparation Note
In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Canu, N., et al. 2005. J. Neurochem.92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.
Beugnet, A., et al. 2003. Biochem. J.372, 555.
Eskelinen, E.L., et al. 2002. Traffic3, 878.
Petiot, A., et al. 2000. J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.
Beugnet, A., et al. 2003. Biochem. J.372, 555.
Eskelinen, E.L., et al. 2002. Traffic3, 878.
Petiot, A., et al. 2000. J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Wei Huang et al.
Frontiers in neuroscience, 15, 745815-745815 (2021-12-07)
Parkinson's disease is a neurodegenerative disorder with an inflammatory response as the core pathogenic mechanism. Previous human genetics findings support the view that the loss of TREM2 function will aggravate neurodegeneration, and TREM2 is one of the most highly expressed
Jinghui Zhai et al.
Journal of biochemical and molecular toxicology, 35(11), e22896-e22896 (2021-08-24)
The NOD-like receptor family pyrin domain-containing (NLRP3) inflammasomes is centrally implicated in cisplatin (CP)-induced kidney injury. Autophagy is critical for inhibiting production of NLRP3 protein that effectively reduces the inflammatory response. Ginsenoside Rg3 (SY), an active component extracted from ginseng
Cong Chen et al.
Cell death & disease, 13(2), 150-150 (2022-02-16)
Ferroptosis, which is characterized by intracellular iron accumulation and lipid peroxidation, is a newly described form of regulated cell death that may play a key role in tumour suppression. In the present study, we investigated the expression profiles and biological
Nienke Visser et al.
International journal of molecular sciences, 22(5) (2021-03-04)
Elevated activation of the autophagy pathway is currently thought to be one of the survival mechanisms allowing therapy-resistant cancer cells to escape elimination, including for cytarabine (AraC)-resistant acute myeloid leukemia (AML) patients. Consequently, the use of autophagy inhibitors such as
Abdelhabib Semlali et al.
Frontiers in oncology, 12, 873447-873447 (2022-10-04)
Treatment of oral cancer is based exclusively on surgery combined with or without chemotherapy. However, it has several side effects. Targeting a new, more effective therapy has become an urgent matter. The purpose of this study was to evaluate the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service