1.50622

Purospher^®STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 100 mm x 4.6 mm Hibar^® RT HPLC Column

• Synonym(s): Purospher^® STAR RP-18 endcapped, 5µm Hibar^® RT 100-4.6
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: C18 (RP18), C18 bonding phase
• Pore size: 120 Å pore size
• Matrix: fully porous particle

Properties

• Product Name: Purospher^® STAR RP-18 endcapped (5 µm), L × I.D. 100 mm x 4.6 mm Hibar^® RT HPLC column
• material: stainless steel column
• Quality Segment: 100
• agency: suitable for USP L1
• product line: Purospher^®
• feature: endcapped
• analyte functional class(es): antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins
• analyte chemical class(es): PAHs, PCBs (aroclor identification), PFAS, aflatoxins, alcohols / fermentation, alcohols and aldehyde, aliphatic amines, alkaloids, amino acids, aromatic amines, cannabinoids as tms derivatives, carotenoids, catecholamines, coloring compounds, essential oils, esters, fatty acids, flavonoids, glycans, ketones, metabolized steroids, nitrosamines, nucleic acids, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides
• packaging: 1 ea of
• extent of labeling: 17% Carbon loading
• parameter: 400 bar pressure, 65 °C max. temp.
• technique(s): HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
• column L × I.D.: 10 cm × 4.6 mm
• matrix: fully porous particle
• matrix active group: C18 (RP18), C18 bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: reversed phase, supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

Purospher^® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher^® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher^® STAR RP-18 endcapped, 5 µm Hibar^® RT 100-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 100 mm length x 4.6 mm I.D. is the optimal choice for the high resolution HPLC separation of complex samples.The recommended guard column is Purospher^® STAR RP-18 endcapped (5 µm) LiChroCART^™ 4-4 part number 1.50250.0001. For direct connection to a Hibar^® RT columns the Precolumn holder for 4-4 LiChroCART^™ cartridges, part number 1.51487.0001 can be used.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 55 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 3.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany