1.25080
Fractogel® EMD DMAE (M)
MiniChrom Fractogel® DMAE (M), volume 5 mL, prepacked column
Synonym(s):
MiniChrom Column Fractogel® DMAE (M), 5 ml
Select a Size
| Size/SKU | Availability | Price |
|---|
About This Item
ligand
diethyl-(2-hydroxypropyl)aminoethyl
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure, ~170 cm/hr max. flow rate
column I.D. × L
8 mm × 100 mm
volume
5 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~100 mg, BSA binding capacity
application(s)
gene therapy
recombinant protein
vaccine development
separation technique
anion exchange
storage temp.
15-25°C
General description
Features and Benefits
- Excellent binding to large viruses and plasmid DNA
- Homogenous binding with high selectivity and purity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| volume 5 mL | volume 5 mL | volume 5 mL | volume 5 mL |
| ligand diethyl-(2-hydroxypropyl)aminoethyl | ligand (Trimethylammoniumethyl) | ligand carboxymethyl | ligand iminodiacetic acid |
| separation technique anion exchange | separation technique anion exchange | separation technique anion exchange | separation technique affinity |
| form prepacked column | form prepacked column | form prepacked column | form prepacked column |
| material polypropylene housing | material polypropylene housing | material polypropylene housing | material polypropylene housing |
| capacity ~100 mg, BSA binding capacity | capacity ~180 mg, BSA binding capacity | capacity ~100 mL, resin binding capacity | capacity ~60 mL, resin binding capacity |
Still not finding the right product?
Explore all of our products under Fractogel® EMD DMAE (M)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.