P7777
Poly(ethylene glycol)
Hybri-Max™, mol wt 1,300-1,600, waxy solid, BioReagent, suitable for hybridoma
Synonym(s):
PEG
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About This Item
Linear Formula:
H(OCH2CH2)nOH
CAS Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:
NACRES:
NA.75
Recommended Products
biological source
synthetic (organic)
Quality Level
grade
Hybri-Max™
sterility
autoclaved
product line
BioReagent
form
waxy solid
mol wt
1,300-1,600
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
viscosity
25-35 cP (210 °F)
mp
40-50 °C
solubility
water: soluble (5 mL water/vial at 45 °C)
SMILES string
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
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Application
Recommended for cell fusion.
Reconstitution
Melt the PEG at 45 °C or higher in a waterbath. Dilute to 30-50% using sterile, pre-warmed serum-free medium (e.g., for a 50% solution add 5 mL to the 5 g vial of PEG). The pH of the solution should be slightly alkaline (pink, not orange or purple). Adjustment of the pH with sterile NaOH may be required. Store solution below 0 °C. Prolonged storage or repeated freezing and thawing is not recommended.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
281.5 °F - closed cup
Flash Point(C)
138.6 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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