Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

I3019

Sigma-Aldrich

Interleukin-10 from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

IL-10

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00212207
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.01-1.50 ng/mL EC50

mol wt

~18 kDa

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P18893

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Il10(16153)

Application

Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Biochem/physiol Actions

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein

Analysis Note

The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCK9359
SLCK1822
SLCH8477
SLCH9276
SLCH4946

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Go Kuwahara et al.
PloS one, 9(9), e106987-e106987 (2014-09-10)
Novel cell therapy is required to treat critical limb ischemia (CLI) as many current approaches require repeated aspiration of bone marrow cells (BMCs). The use of cultured BMCs can reduce the total number of injections required and were shown to
Sergio M Borghi et al.
Experimental physiology, 100(5), 531-544 (2015-02-26)
What is the central question of this study? This study investigated the role of the endogenous anti-inflammatory cytokine interleukin-10 in intense acute swimming-induced muscle mechanical hyperalgesia in mice. What is the main finding and its importance? Endogenous interleukin-10 has a
Philipp Lichte et al.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 200, 73-78 (2015-03-25)
Musculoskeletal injuries induce systemic inflammation which often impairs lung function contributing to morbidity. IL-10 has been shown to have a beneficial effect on immune dysfunction and organ damage after different traumatic insults. We sought to investigate the effect of inhalative
Yoshiyuki Goto et al.
Scientific reports, 5, 15918-15918 (2015-11-03)
Fucosylated glycans on the surface of epithelial cells (ECs) regulate intestinal homeostasis by serving as attachment receptors and a nutrient source for some species of bacteria. We show here that epithelial fucosylation in the ileum is negatively regulated by IL-10-producing
Heather M McGee et al.
Immunology and cell biology, 89(7), 767-776 (2011-01-19)
Neonates have a developing immune response, with a predisposition towards induction of tolerance. As the immune system develops, immunity rather than tolerance is induced, with this development of immunity occurring in response to external factors such as the environment. As
View All Related Papers

Articles

Interleukin Family (IL)

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Interleukin Family (IL)

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Interleukin Family (IL)

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Interleukin Family (IL)

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service