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SAB5801066

Sigma-Aldrich

Alpaca Anti-Goat IgG(Fcγ) HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(s):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

alpaca

recombinant

expressed in E. coli

conjugate

horse radish peroxidase (HRP)

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

recombinant monoclonal

form

lyophilized solid

mol wt

14 kDa

species reactivity

goat, Alpaca

technique(s)

ELISA: 1:5000-1:20000
western blot: 1:5000-1:20000

size

0.1 mL

color

colorless

isotype

VHH

shipped in

wet ice

storage temp.

2-8°C

General description

Alpaca Anti-Goat IgG(Fcγ) HRP single domain Antibody is designed to specifically detect Fc region of goat IgG. It is a monovalent, single domain, recombinant antibody to goat IgG(Fcγ Fragment specific) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Goat IgG(Fcγ) HRP single domain Antibody reacts with the Fc region of goat IgG selectively but not with mouse, rabbit, human, cynomolgus, rat IgG.
Goat antibodies are commonly used in biotechnology. They are used to prepare diagnostic reagents of immunochemical techniques. Goat IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small (less than 15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Fc region of goat IgG
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Other Notes

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG

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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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