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Key Documents

R0406

Sigma-Aldrich

Anti-RUNX1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Acute myeloid leukemia 1 protein, AML-1, Anti-CBF-α2, Anti-Core-binding Factor α2 subunit, Anti-Oncogne AML-1, Anti-PEBP2-αB, PEA2-αB, Anti-Polyomavirus enhancer-binding protein 2αB subunit, Anti-Runt-related transcription factor 1, Anti-SL3-3 enhancer factor 2αB subunit, Anti-SL3/AKV core-binding factor αB subunit

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~48 kDa

species reactivity

human, mouse, rat (predicted)

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed HeLa cells
western blot: 0.25-0.5 μg/mL using HeLa cell lysate

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RUNX1(861)
mouse ... Runx1(12394)
rat ... Runx1(50662)

General description

RUNX1 encodes a DNA-binding subunit.
Runt-related transcription factor 1 (RUNX1) is a transcription factor that plays an important role in hematopoiesis, osteogenesis and neurogenesis. It is a member of Runt-related transcription factors (RUNXs). It was first identified as a component of Polyomavirus enhancer binding protein 2 (PEBP2) and Moloney murine leukemia virus enhancer core binding factor (CBF). Three isoforms of the protein have been identified: RUNX1a, RUNX1b and RUNX1c. Structurally, it consists of Runt domain at the N-terminal region.

Immunogen

synthetic peptide corresponding to amino acids 42-52 of human RUNX1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-RUNX1 (N-terminal) antibody produced in rabbit is suitable for indirect immunofluorescence and western blot.

Biochem/physiol Actions

RUNX activity is regulated by extracellular signaling pathways resulting in post-translational modifications, such as phosphorylation, acetylation and ubiquitination. It is linked to acute leukemia.
RUNX1 (Runt-related transcription factor 1) plays a vital role in tumour suppression and oncogenic activities. During hematopoiesis, it is involved in hematopoietic development, hematopoietic stem cell homeostasis, and various blood malignancies. It may have clinicopathological impact on the proliferation of human bone marrow cells used for transplantation therapy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phosphorylation, acetylation and ubiquitination: the molecular basis of RUNX regulation
Bae SC and Lee YH
Gene, 366(1), 58-66 (2006)
Takako Yokomizo-Nakano et al.
Cancer research, 80(12), 2523-2536 (2020-04-29)
RUNX3, a RUNX family transcription factor, regulates normal hematopoiesis and functions as a tumor suppressor in various tumors in humans and mice. However, emerging studies have documented increased expression of RUNX3 in hematopoietic stem/progenitor cells (HSPC) of a subset of
Nicola Ferrari et al.
PloS one, 9(6), e100759-e100759 (2014-06-27)
The RUNX1 transcription factor is widely recognised for its tumour suppressor effects in leukaemia. Recently a putative link to breast cancer has started to emerge, however the function of RUNX1 in breast cancer is still unknown. To investigate if RUNX1
Kentson Lam et al.
Frontiers in bioscience (Landmark edition), 17, 1120-1139 (2011-12-29)
RUNX1 is a transcription factor that regulates critical processes in many aspects of hematopoiesis. RUNX1 is also integral in defining the definitive hematopoietic stem cell. In addition, many hematological diseases like myelodysplastic syndrome and myeloproliferative neoplasms have been associated with

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