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P2783

Sigma-Aldrich

Phosphotransacetylase from Bacillus stearothermophilus

lyophilized powder, ≥3,000 units/mg protein (biuret)

Synonym(s):

Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase

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About This Item

CAS Number:
9029-91-8
Enzyme Commission number:
2.3.1.8 (BRENDA, IUBMB)
EC Number:
232-855-2
MDL number:
MFCD00131907
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥3,000 units/mg protein (biuret)

composition

Protein, ≥20%

shipped in

wet ice

storage temp.

−20°C

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Application

Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate. It is used to study metabolic pathways in various bacterium.

Biochem/physiol Actions

Phosphotransacetylase converts CoA to acetyl CoA.

Unit Definition

One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.

Physical form

Lyophilized powder containing potassium phosphate

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate
Philip R. Mallinder and Anne Moir
Microbiology, 137, 779-785 (1991)
Ling Jiang et al.
Biotechnology and bioengineering, 108(1), 31-40 (2010-09-09)
Repeated fed-batch fermentation of glucose by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor (FBB) was successfully employed to produce butyric acid at a high final concentration as well as to adapt a butyric-acid-tolerant strain. At the end of the
Federico P Bologna et al.
Journal of microbiology (Seoul, Korea), 48(5), 629-636 (2010-11-04)
The Escherichia coli genes pta and eutD encode proteins containing the phosphate-acetyltransferase domain. EutD is composed only by this domain and belongs to the ethanolamine operon. This enzyme has not been characterized yet, and its relationship to the multimodular E.
Maria Dimou et al.
Molecular biology reports, 39(4), 4135-4143 (2011-07-21)
Cyclophilins belong to the peptidyl-prolyl cis/trans isomerase family of enzymes (EC 5.2.1.8), which accelerate protein folding by catalysing the cis/trans isomerisation of proline imidic peptide bonds. In the present study, by a combination of bioinformatics methods, we identify phosphate acetyltransferase
Jing Huang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(4), 480-487 (2011-07-30)
To study the effects of gene pta disruption on biosynthesis of L-tryptophan. The pta gene of the L-tryptophan producing strain E. coli TRTH was disrupted by Red recombination technology and a pta mutant E. coli TRTHdeltapta was constructed. Fed-batch fermentation
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Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

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