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G9771

Sigma-Aldrich

Grace′s Insect Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

Quality Level

quality

With L-glutamine, without sodium bicarbonate

technique(s)

cell culture | insect: suitable

suitability

suitable for several dipteran and lepidopteran cell lines

components

L-glutamine: 0.6 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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Application

Grace′s medium was originally formulated to support the growth of cells derived from the Australian emperor gum moth, Antherea eucalypti. The medium is a modification of Wyatt′s medium to more closely resemble Antherea hemolymph. The cell lines established by Grace using this medium were the first continuous lines developed. The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects including several species of lepidopterans as well as some dipterans. The medium is primarily used as a basal medium for the growth and maintenance of cell lines derived from lepidopterans.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Salvador Hernández-Martínez et al.
PloS one, 12(4), e0175759-e0175759 (2017-04-21)
Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT
Renjun Tu et al.
Current biology : CB, 31(4), 827-839 (2020-12-29)
The niche controls stem cell self-renewal and progenitor differentiation for maintaining adult tissue homeostasis in various organisms. However, it remains unclear whether the niche is compartmentalized to control stem cell self-renewal and stepwise progeny differentiation. In the Drosophila ovary, inner
Zhenru Zhou et al.
Current biology : CB, 29(5), 856-864 (2019-02-26)
Formation of correctly shaped organs is vital for normal function. The Drosophila wing has an elongated shape, which has been attributed in part to a preferential orientation of mitotic spindles along the proximal-distal axis [1, 2]. Orientation of mitotic spindles
Mauricio Rv Sant'anna et al.
Parasites & vectors, 2(1), 62-62 (2009-12-17)
Leishmania parasites must overcome several barriers to achieve transmission by their sand fly vectors. One of the earliest threats is exposure to enzymes during blood meal digestion. Trypsin-like enzymes appear to be detrimental to parasite survival during the very early
Megan Levis et al.
Biomicrofluidics, 13(2), 024111-024111 (2019-05-09)
Microfluidic devices provide a platform for analyzing both natural and synthetic multicellular systems. Currently, substantial capital investment and expertise are required for creating microfluidic devices using standard soft-lithography. These requirements present barriers to entry for many nontraditional users of microfluidics

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