45125
Elastase from porcine pancreas
lyophilized, powder, white, ~8 U/mg
Synonym(s):
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
biological source
Porcine pancreas
Quality Level
form
powder
quality
lyophilized
specific activity
~8 U/mg
mol wt
Mr ~25000
impurities
salt, none detected
color
white
storage temp.
−20°C
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Biochem/physiol Actions
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Other Notes
For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds
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I Grunnet et al.
The Biochemical journal, 209(1), 215-222 (1983-01-01)
Fatty acid synthetase from goat mammary gland was subjected to limited proteolysis by trypsin and elastase. Both proteolytic enzymes selectively cleaved the chain-terminating thioester hydrolase component from the enzyme complex, leaving all other partial activities intact in the core peptides.
T. Sakurai et al.
Journal of the American Chemical Society, 110, 7236-7236 (1988)
Etsuko Kurimoto et al.
Respiratory research, 14, 5-5 (2013-01-22)
Pulmonary emphysema is characterized by alveolar destruction and persistent inflammation of the airways. Although IL-17A contributes to many chronic inflammatory diseases, it's role in the inflammatory response of elastase-induced emphysema remains unclear. In a model of elastase-induced pulmonary emphysema we
Gerwin E Engels et al.
Journal of cardiothoracic surgery, 8, 4-4 (2013-01-11)
Despite continuous improvements in materials and perfusion techniques, cardiac surgery still causes lung injury and a delay of pulmonary recovery. Currently, there is no gold standard for quantifying cardiac surgery induced lung injury and dysfunction. Adding objective measures, such as
Paul D Thornton et al.
Macromolecular rapid communications, 34(3), 257-262 (2013-01-05)
The generation of a range of star-shaped block copolymers composed of a biocompatible poly(ethylene glycol) (PEG) core tethered to a polyalanine (PAla) shell that possesses the capability to (reversibly) self-assemble in water is described. The hydrogels formed offer a hydrophilic
Chromatograms
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