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11012001

FLO-1 cell line human

Synonym(s):

FLO1 Cells

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About This Item

UNSPSC Code:
41106514

biological source

human esophagus

Quality Level

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

P53 mutation c.830G>T

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Distal oesophageal adenocarcinoma

Application

For complete product information, please see ECACC

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S J Hughes et al.
Cancer research, 57(24), 5571-5578 (1998-01-04)
This study describes Fas (CD95) expression in Barrett's esophagus, adenocarcinomas of the esophagus, and three esophageal adenocarcinoma cell lines. Immunohistochemical analysis of Barrett's esophagus demonstrated cell surface expression of Fas protein. In contrast, 30.5% of esophageal adenocarcinomas examined by immunohistochemical
Mathieu Francois Derouet et al.
PloS one, 9(12), e115589-e115589 (2015-01-01)
Carcinoma of the esophagus has a high case fatality ratio and is now the 6th most common cause of cancer deaths in the world. We previously conducted a study to profile the expression of miRNAs in esophageal adenocarcinoma (EAC) pre
Sarah Derks et al.
Cancer immunology research, 3(10), 1123-1129 (2015-06-18)
Esophageal adenocarcinoma is an increasingly common disease with a dismal 5-year survival rate of 10% to 15%. In the first systematic evaluation of the PD-1 pathway in esophageal adenocarcinoma, we identify expression of PD-L2 in cancer cells in 51.7% of
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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