17170

AEA Sporulation Broth (Base), modified

NutriSelect^® Plus, suitable for microbiology

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: ammonium acetate, 1.5 g/L ; biopeptone, 10 g/L ; disodium phosphate, 4.36 g/L ; magnesium sulfate.7H₂O, 0.2 g/L ; monopotassium phosphate, 0.25 g/L ; yeast extract, 10 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.8±0.2 (25 °C)
• application(s): food and beverages; microbiology
• suitability: nonselective for Clostridium spp.

Description

Application

For early sporulation of Clostridium perfringens from foods.

Preparation Note

Suspend 26.31 g in 1 litre distilled water. Dispense the medium in 15 ml amounts in screw capped tubes and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 0.6 ml of filter sterilized 10% raffinose and 0.2 ml each of sterile 0.66 M sodium carbonate and 0.32% cobalt chloride dropwise to each 15 ml base medium in the tubes. Just before use, steam the medium for 10 minutes and after cooling, add 0.2 ml of filter sterilized (freshly prepared) 1.5% sodium ascorbate to each tube of the medium.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves