MAB3118
Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11
ascites fluid, clone 2EL-1B11, Chemicon®
Synonym(s):
Anti-FLAG Antibody, FLAG Tag Antibody
About This Item
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2EL-1B11, monoclonal
species reactivity
vertebrates, E. coli
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
General description
FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Specificity
Immunogen
Application
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.
Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.
ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.
Immunoblotting:
on recombinant proteins
Optimal working dilutions must be determined by the end user.
Epitope Tags & General Use
Epitope Tags
Quality
Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
Linkage
Physical form
Storage and Stability
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Cells transfected with FLAG-tagged fusion vector
Other Notes
Legal Information
Disclaimer
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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