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EF5011

Sigma-Aldrich

Anti-EF5 Antibody, clone ELK3-51 Antibody, Cyanine 3 conjugate

clone ELK3-51, from mouse, CY3 conjugate

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

biological source

mouse

conjugate

CY3 conjugate

antibody product type

primary antibodies

clone

ELK3-51, monoclonal

species reactivity

rat, mouse, human

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG1

General description

A number of imaging markers which target hypoxia exist and are widely used in research. EF5 (2-(2-Nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide) is a compound developed at the University of Pennsylvania by Dr. Cameron Koch and Dr. Sydney Evans. Upon injection into animal tissues, EF5 selectively binds to hypoxic cells and forms adducts. A mouse monoclonal antibody, clone ELK3-51, which is directly conjugated to Cyanine 3, is then used to selectively bind the EF5 adducts providing a sensitive and quantitative method to detect and measure tissue hypoxia in animal and human tumors, normal tissues and cells.

Advantages of the EF5 hypoxia detection method:
• EF5 exists in only one form; Pimidozole, an alternative hypoxia marker, exists in two forms; one of which is charged and very hydrophilic, the other lipophilic. Pimidozole thus has a very complex biodistribution. In contrast, EF5 is lipophilic and uncharged and this allows very rapid and even tissue distribution.
• EF5 binding images can be calibrated to provide quantitative data on the pO2 values of each cell (1). The fluorescent images obtained from EF5 binding can be calibrated according to camera settings and a “cube-binding” value which is obtained through a separate procedure. The intensity values of calibrated images are directly related to actual tissue pO2 values. As a result, these images provide information regarding not only where hypoxic areas may or may not be, but also data regarding the distribution and levels of hypoxia.

Reference:
Koch CJ (2002) Measurement of absolute oxygen levels in cells and tissues using oxygen sensors and 2-nitroimidazole EF5. Methods in Enzymology 352: 3-31.




Application

Anti-EF5 Antibody, clone ELK3-51 Antibody, Cyanine 3 conjugate is provided in a concentrated format at 2 mg/mL. For optimal performance, antibody should be diluted to a working concentration of at least 75 ug/mL. Refer to product datasheet for details.
Research Category
Cancer
Research Sub Category
Hypoxia
The EF5 hypoxia detection method provides a sensitive and quantitative method to detect and measure tissue hypoxia in animal and human tumors, normal tissues and cells.

Storage and Stability

Store at -20°C.

Other Notes

Concentration: Please refer to product datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Brennan J Wadsworth et al.
British journal of cancer, 126(11), 1616-1626 (2022-01-16)
Solid tumour perfusion can be unstable, creating transiently hypoxic cells that can contribute to radiation resistance. We investigated the in vivo lifetime of transiently hypoxic tumour cells and chronically hypoxic tumour cells during tumour growth and following irradiation. Hypoxic cells

Articles

Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.

Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.

Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.

Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.

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