Skip to Content
Merck
All Photos(1)

Key Documents

AP183P

Sigma-Aldrich

Goat Anti-Rat IgG Antibody, HRP conjugate, Species Adsorbed

0.8 mg/mL, Chemicon®

Synonym(s):

Goat IgG Antibody

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

concentration

0.8 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

Application

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.
Goat anti-Rat IgG Antibody, HRP conjugate, Species Adsorbed detects level of Rat IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Linkage

Replaces: AP306P

Physical form

Lyophilized. Buffer = 0.01 M sodium phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Su-Kyeong Hwang et al.
Molecular brain, 9(1), 56-56 (2016-05-25)
Tuberous sclerosis complex (TSC) is a neurocutaneous disorder characterized by multiple symptoms including neuropsychological deficits such as seizures, intellectual disability, and autism. TSC is inherited in an autosomal dominant pattern and is caused by mutations in either the TSC1 or
Sodium-level-sensitive sodium channel Na(x) is expressed in glial laminate processes in the sensory circumventricular organs.
Watanabe, E; Hiyama, TY; Shimizu, H; Kodama, R; Hayashi, N; Miyata, S; Yanagawa, Y; Obata et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null
Tyler Milston Renner et al.
Bio-protocol, 8(17), e3005-e3005 (2018-09-05)
There exists a wide variety of techniques to isolate and purify viral particles from cell culture supernatants. However, these techniques vary greatly in ease of use, purity, yield and impact on viral structural integrity. Most importantly, it is becoming evident
Kimberly A Edgel et al.
Biochimica et biophysica acta, 1821(3), 425-434 (2011-12-20)
The prevalence of obesity has reached epidemic proportions and is associated with several co-morbid conditions including diabetes, dyslipidemia, cancer, atherosclerosis and gallstones. Obesity is associated with low systemic inflammation and an accumulation of adipose tissue macrophages (ATMs) that are thought
Luisa Martinez-Pomares et al.
Methods in molecular biology (Clifton, N.J.), 415, 255-272 (2008-03-29)
In this chapter, we describe current protocols used for the characterization of macrophages (MPhi) in mouse tissues and in cell suspensions from spleen and lymph nodes. Also, we include a brief description of a complementary approach: culture of primary MPhi.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service