AB3080P
Anti-Green Fluorescent Protein Antibody
Chemicon®, from rabbit
Synonym(s):
GFP, eGFP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
vertebrates (most common)
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Specificity
AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
Reactivity with other species has not been confirmed.
Immunogen
Highly purified native GFP from Aequorea victoria.
Application
Anti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western Blot:
A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.
ELISA:
A 1:800-1:1,000 dilution of a previous lot was used in ELISA.
Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore′s highly purified secondary antibodies in indirect detection systems.
Immunocytochemistry:
Optimal working dilutions and protocols must be determined by end user.
A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.
ELISA:
A 1:800-1:1,000 dilution of a previous lot was used in ELISA.
Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore′s highly purified secondary antibodies in indirect detection systems.
Immunocytochemistry:
Optimal working dilutions and protocols must be determined by end user.
Quality
Routinely evaluated by immunocytochemistry on RENCX cells.
Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot′s anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot′s anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
Target description
Varies (GFP is 27 kDa)
Physical form
Affinity purified rabbit immunoglobulin
Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer.
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Analysis Note
Control
Cells transfected with GFP-tagged fusion vector
RENCX cells
Cells transfected with GFP-tagged fusion vector
RENCX cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Wanying Zhang et al.
eLife, 8 (2019-02-12)
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Devergne, O; Tsung, K; Barcelo, G; Schupbach, T
Proceedings of the National Academy of Sciences of the USA null
Colleen E Hannon et al.
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Jason Seth Rothman et al.
eLife, 5 (2016-08-20)
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Olivier Devergne et al.
Cell reports, 18(8), 1831-1839 (2017-02-24)
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