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262015

Sigma-Aldrich

DNA Base Excision Repair Pathway Inhibitor

The DNA Base Excision Repair Pathway Inhibitor, also referenced under CAS 6960-45-8, controls the biological activity of APE1 (human apurinic/apyrimidinic endonuclease.

Synonym(s):

DNA Base Excision Repair Pathway Inhibitor, 7-Nitroindole-2-carboxylic acid, CRT0044876

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About This Item

Empirical Formula (Hill Notation):
C9H6N2O4
CAS Number:
Molecular Weight:
206.15
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.54

Quality Level

Assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

tan

solubility

DMSO: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)/p-1

InChI key

BIUCOFQROHIAEO-UHFFFAOYSA-M

General description

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC50 = ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H2O2 in HeLa, HT1080, and MDA-MB-231 cells.
A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC50 ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H2O2 in HeLa, HT1080, and MDA-MB-231 cells.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
APE 1
Product does not compete with ATP.
Reversible: no
Target IC50: ~3 µM against APE1 (human apurinic/apyrimidinic endonuclease

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Madhusudan, S., et al. 2005. Nucl. Acids Res.33, 4711.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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