1.50449
SeQuant™ ZIC®-HILIC 200Å (3.5 µm) HPLC Columns
L × I.D. 150 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel
Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
About This Item
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product name
SeQuant™ ZIC-HILIC™ 3.5µm,200Å 150 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
Agency
suitable for USP L114
product line
SeQuant™
feature
endcapped: no
parameter
500 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
15 cm × 4.6 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
Thanks to their favorable retention and robust performance, SeQuant™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant™ ZIC®-HILIC for analysis of melamine and related substances. SeQuant™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
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