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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G11.10.5, monoclonal
mol wt
calculated mol wt 10.21 kDa
observed mol wt ~8.0 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2aκ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
mouse ... CCL4(6351)
General description
C-C motif chemokine 4 (UniProt: P13236; also known as G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, LAG-1, MIP-1-beta (1-69),Macrophage inflammatory protein 1-beta, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, ACT-2) is encoded by the CCL (also known as LAG1, MIP1B, SCYA4) gene (Gene ID: 6351) in human. CCL4/MIP1b is a secretory protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature form (aa 24-292) that can be further proteolytically cleaved at the N-terminal to generate MIP-1b (aa 26-92). It is present either as a homodimer or a heterodimer of MPIa and MIP1b. Both MIP-1a and MIP1β participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. Although they exert similar effects on monocytes their effect on lymphocytes differs; with MIP1a selectively attracting CD8+ lymphocytes and MIP1β selectively attracting CD4+ lymphocytes. MIP1b serves as a monokine with inflammatory and chemokinetic properties. It is one of the major HIV-suppressive factors produced by CD8+ T-cells that inhibits different strains of HIV-1, HIV-2, and simian immunodeficiency virus. CCR5 serves as a specific receptor for MIP1b. The processed form MIP-1b also retains the ability to induce down-modulation of surface expression of the chemokine receptor CCR5 and inhibit the CCR5-mediated entry of HIV-1 in T-cells. It also serves as a ligand for CCR1 and CCR2 isoform B.
Specificity
Clone 4G11.10.5 is a mouse monoclonal antibody that detects human C-C motif chemokine 4 (CCL4/MIP1b ).
Immunogen
Full-length human recombinant C-C-motif chemokine 4 (CCL4) (without the signal peptide).
Application
Quality Control Testing
Evaluated by Western Blotting with human recombinant C-C motif chemokine 4 (CCL4).
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human CCL4/MIP1b .
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected CCL4/MIP1b a in human small intestine and human adrenal gland tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with human recombinant C-C motif chemokine 4 (CCL4).
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human CCL4/MIP1b .
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected CCL4/MIP1b a in human small intestine and human adrenal gland tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CCL4/MIP1β, clone 4G11.10.5, Cat. No. MABF3180, is a mouse monoclonal antibody that detects CCL4/ MIP1β and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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