ABT288
Anti-PICALM
from rabbit, purified by affinity chromatography
Synonym(s):
Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein
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All Photos(6)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... PICALM(8301)
General description
Phosphatidylinositol-binding clathrin assembly protein (UniProt: Q13492; also known as PICALM, Clathrin assembly lymphoid myeloid leukemia protein) is encoded by the PICALM (also known as CALM) gene (Gene ID: 8301) in human. PICALM is ubiquitously expressed protein that co-localizes with clathrin in the Golgi area. Five isoforms of PICALM have been reported that are produced by alternative splicing. PICALM acts as an assembly protein that recruits clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. It may also play a role in determining the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. It is reported to be involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. PICALM variants are associated with Alzheimer′s disease and are thought to be involved in the endocytosis and recycling of the amyloid protein precursor and it can affect Alzheimer s disease disk primarily by modulating production, transportation, and clearance of beta-amyloid peptides. (Ref.: Xu, W., et al. (2015). Mol Neurobiol. 52(1);399-413).
Specificity
This rabbit polyclonal antibody detects Phosphatidylinositol-binding clathrin assembly protein (PICALM) in human cells. it targets an epitope within 16 amino acids from the C-terminal half. It recognizes all 5 isoforms of PICALM.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Phosphatidylinositol-binding clathrin assembly protein (PICALM).
Application
Anti-PICALM Antibody, Cat. No. ABT288, is a rabbit polyclonal ntibody that detects PICALM in nhuman cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Cell Structure
Cell Structure
Western Blotting Analysis: A 1:500 dilution from a representative lot detected PICALM in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in MOG-G-UVW human astrocytoma cell line (Courtesy of Dr Rhian Thomas from Cardiff University).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot PICALM in Mog-G-UVW human astrocytoma cell lysate and HeLa cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in Human H4 neuroglioma cell lysate and SH-SY5Y neuroblastoma cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University).
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PICALM in human colon and human kidney tissue sections.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in MOG-G-UVW human astrocytoma cell line (Courtesy of Dr Rhian Thomas from Cardiff University).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot PICALM in Mog-G-UVW human astrocytoma cell lysate and HeLa cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PICALM in Human H4 neuroglioma cell lysate and SH-SY5Y neuroblastoma cell lysate (Courtesy of Dr Rhian Thomas from Cardiff University).
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PICALM in human colon and human kidney tissue sections.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PICALM in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PICALM in HeLa cell lysate.
Target description
~61 kDa observed; 70.76, 68.76, 66.39, 59.92, and 70.04 kDa calculated, respectively for isoforms 1 to 5. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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