ABE413

Anti-NF-E2-related factor 2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor erythroid derived 2-like 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_006155
• UniProt accession no.: Q16236
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... NFE2L2(4780)

Description

General description

NF-E2-related factor 2 (Nuclear factor erythroid 2-related factor 2) is a transcription activator that binds to antioxidant response elements (ARE) in the promoter regions of target genes. NF-E2-related factor 2 mediates the coordinated up-regulation of genes in response to oxidative stress. NF-E2-related factor 2 may also be involved in the transcriptional activation of genes of the beta-globin cluster. NF-E2-related factor 2 is widely expressed, with highest expression in adult muscle, kidney, lung, liver and fetal muscle.

Specificity

This antibody recognizes the C-terminus of NF-E2 related factor 2

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human NF-E2 related factor 2.

Application

Anti-NF-E2-related factor 2 Antibody is a highly specific rabbit polyclonal antibody, that targets NF-E2-related factor 2 & has been tested in western blotting & ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blotting Analysis: 1 µg/mL from a representative lot detected NF-E2 related factor 2 in 10 µg of Jurkat cell lysate.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected NF-E2 related factor 2 in cells untreated and treated with hydrogen peroxide (Note: Cytoplasmic staining is increased under oxidative stress.).

Quality

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NF-E2 related factor 2 in 10 µg of Ramos cell lysate.

Target description

~75 kDa observed. Two isoforms at 68 kDa (isoform 1) and 66 kDa (isoform 2) are known to exist. This protein has been observed at ~75 kDa due to the abundance of acidic residues found in Nrf2, which causes it to migrate anomalously in SDS-PAGE (Abu-Bakar, A., et al. (2007) Drug Metab Dispos. 35(5):787-794.). A ubiquitinated form of NF-E2-related factor 2 may be observed at ~110 kDa (Li, J., et al. (2005). Toxicol Sci. 83(2):313-328.).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Ramos cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable