647926-M

Trichostatin A, Streptomyces sp.

InSolution, ≥98%, reversible inhibitor of histone deacetylase

• Synonym(s): InSolution Trichostatin A, Streptomyces sp., 4,6-Dimethyl-7-[p-dimethylaminophenyl]-7-oxahepta-2,4-dienohydroxamic Acid, HDAC Inhibitor IX, TSA
• Empirical Formula (Hill Notation): C₁₇H₂₂N₂O₃
• Molecular Weight: 302.37
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• storage temp.: −20°C

Description

General description

A potent and reversible inhibitor of histone deacetylase. Blocks cell cycle progression at the G1 phase in HeLa cells and induces a 12-fold increase in intracellular levels of gelsolin. Induces reversion of oncogenic ras-tranformed NIH 3T3 cells to a normal morphology. Inhibits IL-2 gene expression (IC₅₀ = 73 nM) in Jurkat cells and shows immunosuppressive activity in a mouse model.

Biochem/physiol Actions

Primary Target
histone deactylase

Target IC₅₀: 73 nM inhibiting IL-2 gene expression in Jurkat cells

Warning

Toxicity: Harmful (C)

Physical form

A 10 mM (500 µg/165 µl) solution of Trichostatin A, Streptomyces sp. (Cat. No. 647925) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: 188.6 °F - closed cup - (Dimethylsulfoxide)
• flash_point_c: 87 °C - closed cup - (Dimethylsulfoxide)