Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
Physical form
In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% Brij® 35 detergent, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79. Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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