1.09205

modified Tryptic Soy Broth- Dehydrated Culture Media

GranuCult^® prime, Novobiocin 20 mg/L, ISO 16654, granular, selective for Escherichia coli

• Synonym(s): TSB modified, Tryptic soy broth, modified, mTSB
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: FDA-BAM; ISO 16654
• Quality Level: 200
• description: with Novobiocin (20 mg/L) acc. ISO 16654 GranuCult
• sterility: non-sterile
• form: granular
• composition: Novobiocin, 20 mg/L
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 7.1-7.5 (25 °C, 33 g/L in H₂O, after autoclaving)
• solubility: 33 g/L
• application(s): food and beverages; microbiology; pathogen testing
• storage temp.: 15-25°C
• suitability: Escherichia coli (O157:H7 ); selective for Escherichia coli (EHEC); urea-degrading bacteria

Description

General description

Modified Tryptic Soy Broth with Novobiocin is recommended for the enrichment of enterohemorrhagic E. coli (EHEC). The nutrients in mTSB broth provide favorable growth conditions, while the mixture of bile salts No. 3 and Novobiocin suppress the growth of the Gram-positive microbial flora.

Application

mTSB broth with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli (EHEC) as well as detection of E. coli serotype O157:H7 in foods.

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish-brown
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of E.coli and S.aureus
Additionaly monoculture of S.aureus

Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 yellowish-brown colonies on CT-SMAC agar
Growth (Escherichia coli ATCC 700728 (WDCM 00014)): >10 yellowish-brown colonies on CT-SMAC agar
Growth Escherichia coliATCC 25922 (WDCM 00013): >10 pink colonies on CT-SMAC agar
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): total inhibition on TSA
Incubation:
mTSB broth: 18 - 24 hours at 41,5 °C, aerobic
Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3