1.05222

GranuCult^® plus Kanamycin esculin azide agar

for Enterococcus spp., suitable for microbiology

• Synonym(s): D-Streptococci selective agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 6.9-7.3 (37 °C, 47 g/L in H₂O, after autoclaving)
• solubility: 47.5 g/L
• bulk density: 780 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Enterococcus spp.

Description

General description

GranuCult^® plus Kanamycin esculin azide agar is a highly selective media for D-streptococci. Kanamycin and azide inhibit the accompanying bacterial flora. D-streptococci are, however, only slightly sensitive to these substances, so they grow normally and hydrolyze the glucoside esculin to give glucose and esculetin. Esculetin forms an olive-green to-black complex with iron (III) ions.

Application

GranuCult^® plus Kanamycin esculin azide agar is suitable for the isolation, differentiation, and enumeration of enterococci in foodstuffs, water, and other materials.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): brown-bluish
pH-value (25 °C): 6.9 - 7.3
Recovery on test medium (Enterococcus faecalis ATCC 11700): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Staphylococcus aureus ATCC 6538-P (WDCM 00033)): no limit
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Colour change to olivegreen-black (Enterococcus faecalis ATCC 11700): +
Colour change to olivegreen-black (Enterococcus hirae ATCC 8043 (WDCM 00089)): +
Colour change to olivegreen-black (Enterococcus durans ATCC 6056): +
Colour change to olivegreen-black (Staphylococcus aureus ATCC 6538-P (WDCM 00033)): -
Colour change to olivegreen-black (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Colour change to olivegreen-black (Escherichia coli ATCC 11775 (WDCM 00090)): -
Incubation: 48 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3