1.00215
GranuCult® prime Hektoen Enteric Agar
according to ISO 21567, FDA-BAM, according to ISO 6579, selective and differential for Salmonella spp., selective and differential for Shigella spp., suitable for microbiology
Synonym(s):
HE Agar, HEA, Hektoen Enteric Agar
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.74
Recommended Products
agency
FDA-BAM
according to ISO 21567
according to ISO 6579
Quality Level
sterility
non-sterile
sterilization compatibility
autoclavable compatible
form
granular
packaging
pkg of 500 g
manufacturer/tradename
GranuCult® prime
storage condition
protect from light (Keep dry and tightly closed)
technique(s)
microbiological culture: suitable
color
dark green
pH
7.5-7.9 (25 °C, 75 g/L in H2O)
solubility
75 g/L
application(s)
clinical
food and beverages
industrial qc
pharmaceutical
microbiology
storage temp.
15-25°C
suitability
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
General description
GranuCult® prime Hektoen Enteric Agar provides good colonial differentiation and inhibits some coliforms and other non-lactose-fermenting bacteria, thereby facilitating the isolation and differentiation of Salmonella spp. and Shigella spp. from samples such as food chain and other materials.Lactose-positive colonies have a different color from lactose-negative ones due to the presence of the two indicators for carbohydrate dissimilation: bromothymol blue and acidic fuchsin. The two additional carbohydrates, sucrose (saccharose) and salicin, also cause the color difference in colonies. The black color in the H2S-positive colonies is due to thiosulfate as a reactive compound with an iron salt as an indicator.This culture medium is moderately to highly selective. The mixture of bile salts suppresses the growth of most of the accompanying microorganisms. The inhibitory effect of bile salts on the growth of Shigella spp. is reduced by relatively large amounts of peptone and carbohydrates, which allow good growth. Agar is the solidifying agent.
Application
GranuCult® prime Hektoen Enteric Agar is suitable for the isolation and differentiation ofSalmonella and Shigella from the food chain and other materials.
Features and Benefits
- GranuCult® offers superior granulated culture media.
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
- Excellent wettability, solubility, and free-flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions.
- High batch-to-batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
- Granulation technology allows many supplements to be included, with no need to add these separately
Storage and Stability
Self-prepared plates can be stored in the dark and protected against evaporation:
- acc. to ISO 21567 at (5 ± 3 °C) for up to 2 weeks
- acc. to FDA-BAM at (4 ± 2 °C) for up to 30 days
- acc to Corry et al., (2012) at (5 ± 3 °C) for up to 3 weeks
Analysis Note
- Salmonella spp. produce transparent green to blue-green colonies, with or without black centers, or may appear completely black due to H2S production.
- Shigella spp. produce green, transparent moist colonies.
- Lactose, Sucrose or Salicin fermenting gram-negative bacteria may be inhibited or produce red to salmon colored colonies with or without precipitation zone.
Other Notes
- Do not use clumped or discolored medium.
- The prepared medium is clear to slightly opalescent and blue-green to blue-grey.
- Before inoculation, allow the prepared medium to equilibrate at room temperature if it is stored at a lower temperature.
- There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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