03-0171-00

SMC^® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit

• Synonym(s): Abrineurin immunoassay kit, BDNF Single Molecule Counting Kit, BDNF ultra-sensitive immunoassay
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.04 pg/mL; (lower limit of quantification); inter-assay cv: <11%; intra-assay cv: <10%; ((Intra-assay CV n=20; Inter-assay n=5))
• technique(s): ELISA: suitable; single molecule counting: suitable
• relevant disease(s): Alzheimer′s disease
• storage temp.: 2-8°C

Description

General description

96 well assay

Brain-derived neurotrophic factor (BDNF) is a protein made by neuronal cells critical for the growth and functioning of neurons as well as binding at synapses to improve signal strength between neurons. While sufficient levels of BDNF promote the function and growth of brain cells and neurons, a decrease in BDNF levels can lead to impaired neurological function and is associated with diseases such as Alzheimer′s, epilepsy, schizophrenia and depression.

The SMC^® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Specificity

Cross Reactivity:
Cross reactivity and interference of ProBDNF was tested in this assay.
The assay shows 1.2% cross reactivity with ProBDNF. Serum and plasma spiked with ProBDNF show an average 4.4% interference.

Accuracy:

• Minimum of 5 EDTA plasma samples spiked with average recovery of 96% with a range of 81-108%
• Minimum of 5 serum samples spiked with average recovery of 90% with a range of 71-98%
• Minimum of 5 cerebrospinal fluid (CSF) samples spiked with average recovery of 98% with a range of 83-108%

Application

Research Category: 

• Neuroscience 

Research Sub-Category: 

• Alzheimer′s Disease
• Neurochemistry
• Neurotrophins
• Signaling Neuroscience

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of BDNF in human EDTA plasma, serum, and cerebrospinal fluid.

Features and Benefits

This new kit includes the following features:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Components

• Assay Buffer
• BDNF Coated Beads
• Standard Diluent
• BDNF Detection Antibody
• BDNF Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H373,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects