An Extended-gate Type Organic FET Based Biosensor for Detecting Biogenic Amines in Aqueous Solution.

An Extended-gate Type Organic FET Based Biosensor for Detecting Biogenic Amines in Aqueous Solution.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2015-07-15)

Tsuyoshi Minami, Tsubasa Sato, Tsukuru Minamiki, Shizuo Tokito

RESUMEN

This study is the first to report on the detection of biogenic amines in an aqueous solution using an organic field-effect transistor (OFET) device with an extended gate electrode modified with a layer of diamine oxidase and a horseradish peroxidase osmium-redox polymer. The limit of detection (LOD) for histamine was estimated to be 1.2 μM. These results reveal that extended-gate type OFET devices are highly suitable enzyme-based biosensors for the detection of biogenic amine levels.

MATERIALES

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H3375

Sigma-Aldrich

HEPES, ≥99.5% (titration)

H4034

Sigma-Aldrich

HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

54457

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HEPES, BioUltra, for molecular biology, ≥99.5% (T)

S0266

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Spermidine, BioReagent, for molecular biology, suitable for cell culture, ≥98%

S2626

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Spermidine, ≥99% (GC)

83264

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HEPES buffer solution, 1 M in H₂O

85558

Sigma-Aldrich

Spermidine, BioUltra, for molecular biology, ≥99.5% (GC)

RES6003H-B7

SAFC

HEPES

H7250

Sigma-Aldrich

Histamine dihydrochloride, ≥99% (TLC), powder

D13208

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1,4-Diaminobutano, 99%

H6147

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HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

T2879

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Tyramine hydrochloride, ≥98%

D22606

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Cadaverine, 95%

PHG0001

SAFC

HEPES

S1141

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Spermine tetrahydrochloride, BioReagent, for molecular biology

G5882

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Glutaraldehyde solution, Grade I, 25% in H₂O, specially purified for use as an electron microscopy fixative

53300

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Histamine dihydrochloride, ≥99.0% (AT)

340855

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Glutaraldehyde solution, 50 wt. % in H₂O

G6257

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Glutaraldehyde solution, Grade II, 25% in H₂O

85605

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Spermine tetrahydrochloride, BioUltra, for molecular biology, ≥99.5% (AT)

H7523

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HEPES, BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

S2876

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Spermine tetrahydrochloride, powder or crystals

W512303

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Glutaric dialdehyde solution, 50 wt. % in H₂O, FCC

736414

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Tetradecylphosphonic acid, 98%

240664

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1,2-Dichlorobenzene, anhydrous, 99%

263257

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Osmium, powder, 99.9% trace metals basis

G7776

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Glutaraldehyde solution, Grade I, 70% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

RDD002

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HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%

G7526

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Glutaraldehyde solution, Grade I, 8% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

G7651

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 50% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use